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Transgene-free genome editing in poplar.杨树中的无转基因基因组编辑
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Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy.通过共编辑策略在 T0 代中对营养繁殖和多年生植物物种进行无转基因基因组编辑。
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Evaluating the Efficiency of gRNAs in CRISPR/Cas9 Mediated Genome Editing in Poplars.评估 gRNAs 在杨树 CRISPR/Cas9 介导的基因组编辑中的效率。
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Efficient Genome Editing in Using CRISPR/Cas12a.利用CRISPR/Cas12a在[具体对象]中进行高效基因组编辑。 （你原文中“in”后面缺少具体内容，我按照合理补充后的形式翻译了）
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Advances in genomics and genome editing for breeding next generation of fruit and nut crops.用于培育下一代水果和坚果作物的基因组学与基因组编辑进展。
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CRISPR-Combo-mediated orthogonal genome editing and transcriptional activation for plant breeding.CRISPR-Combo 介导的植物育种正交基因组编辑和转录激活。
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本文引用的文献
1
Transgene-free genome editing in poplar.杨树中的无转基因基因组编辑
New Phytol. 2025 Jul;247(1):224-232. doi: 10.1111/nph.20415. Epub 2025 Jan 22.
2
Binary vector copy number engineering improves Agrobacterium-mediated transformation.二元载体拷贝数工程改进了农杆菌介导的转化。
Nat Biotechnol. 2024 Nov 4. doi: 10.1038/s41587-024-02462-2.
3
Rare but diverse off-target and somatic mutations found in field and greenhouse grown trees expressing CRISPR/Cas9.在表达CRISPR/Cas9的田间和温室种植树木中发现了罕见但多样的脱靶突变和体细胞突变。
Front Bioeng Biotechnol. 2024 Jun 21;12:1412927. doi: 10.3389/fbioe.2024.1412927. eCollection 2024.
4
Social and biological innovations are essential to deliver transformative forest biotechnologies.社会和生物创新对于推动变革性的森林生物技术至关重要。
New Phytol. 2024 Jul;243(2):526-536. doi: 10.1111/nph.19855. Epub 2024 May 27.
5
Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy.通过共编辑策略在 T0 代中对营养繁殖和多年生植物物种进行无转基因基因组编辑。
Nat Plants. 2023 Oct;9(10):1591-1597. doi: 10.1038/s41477-023-01520-y. Epub 2023 Sep 18.
6
Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation.利用 Cas12a/crRNA 核糖核蛋白在 T0 代中生成无转基因溃疡抗性柑橘。
Nat Commun. 2023 Jul 5;14(1):3957. doi: 10.1038/s41467-023-39714-9.
7
Multiplex Editing of the Tandem Array in Poplar: From Small Indels to Translocations and Complex Inversions.杨树木质部串联重复序列的多位点编辑：从小的缺失/插入到易位和复杂倒位。
CRISPR J. 2023 Aug;6(4):339-349. doi: 10.1089/crispr.2022.0096. Epub 2023 Jun 12.
8
Engineered Agrobacterium improves transformation by mitigating plant immunity detection.工程改造的农杆菌通过减轻植物免疫检测来提高转化效率。
New Phytol. 2023 Mar;237(6):2493-2504. doi: 10.1111/nph.18694. Epub 2023 Jan 17.
9
Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation.表达 III 型分泌系统的农杆菌将假单胞菌效应物递送到植物细胞中，以增强转化。
Nat Commun. 2022 May 11;13(1):2581. doi: 10.1038/s41467-022-30180-3.
10
Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA.利用单个 gRNA 对杂种杨树中调控毛状体形成的 MYB 重复基因进行多重敲除。
Plant Physiol. 2022 Jun 1;189(2):516-526. doi: 10.1093/plphys/kiac128.
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