Meltzer P S, Cheng Y C, Trent J M
Cancer Genet Cytogenet. 1985 Aug;17(4):289-300. doi: 10.1016/0165-4608(85)90112-8.
Detailed cytogenetic and molecular biologic studies have been performed on the human KB tumor cell line and four methotrexate-resistant subclones. Results are presented, demonstrating that the gene encoding the target enzyme dihydrofolate reductase is increasingly amplified in progressively methotrexate-resistant subclones, and that dihydrofolate reductase sequences are localized to a homogeneously staining region on chromosome 10q.
已对人KB肿瘤细胞系和四个耐甲氨蝶呤亚克隆进行了详细的细胞遗传学和分子生物学研究。本文展示了研究结果，表明编码靶酶二氢叶酸还原酶的基因在逐渐耐甲氨蝶呤的亚克隆中扩增程度越来越高，且二氢叶酸还原酶序列定位于10号染色体长臂上的一个均匀染色区。
