Korc M, Meltzer P, Trent J
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5141-4. doi: 10.1073/pnas.83.14.5141.
Recently, the gene for the epidermal growth factor (EGF) receptor has been mapped to chromosome 7p, the short arm of chromosome 7 [Shimizu, N., Kondo, I., Gamou, M. A., Behzadian, A. & Shimizu, Y. (1984) Somatic Cell Mol. Genet. 10, 45-53]. Utilizing EGF binding in saturation studies, karyology, and cDNA hybridization experiments, we have sought to determine whether there is a correlation between dosage or alteration of chromosome 7 and enhanced expression of EGF receptor in cultured human pancreatic carcinoma cells. Saturation binding studies with 125I-labeled EGF were performed at 4 degrees C with four established human pancreatic cancer cell lines: T3M4, PANC-1, COLO 357, and UACC-462. Analysis of binding data revealed enhanced numbers of EGF receptors in all four cell lines. Chromosome banding analysis revealed clonal structural alterations of chromosome 7p in the cell lines T3M4, PANC-1, and COLO 357, whereas UACC-462 displayed multiple copies of chromosome 7. Hybridization studies using a radiolabeled EGF receptor cDNA probe failed to demonstrate DNA sequence amplification in any cell line but confirmed the presence of EGF receptor mRNA in these cells in approximate proportion to EGF receptor number. Our results suggest that enhanced expression of EGF receptor in human pancreatic cancer can be associated with either structural or numerical alterations of chromosome 7.
最近，表皮生长因子（EGF）受体基因已被定位到7号染色体的短臂7p上[Shimizu, N., Kondo, I., Gamou, M. A., Behzadian, A. & Shimizu, Y. (1984) Somatic Cell Mol. Genet. 10, 45 - 53]。利用饱和研究中的EGF结合、核型分析和cDNA杂交实验，我们试图确定7号染色体的剂量或改变与培养的人胰腺癌细胞中EGF受体表达增强之间是否存在相关性。用125I标记的EGF对四种已建立的人胰腺癌细胞系：T3M4、PANC - 1、COLO 357和UACC - 462进行了4℃下的饱和结合研究。结合数据分析显示，所有四种细胞系中EGF受体数量均增加。染色体带型分析显示，T3M4、PANC - 1和COLO 357细胞系中7号染色体短臂存在克隆性结构改变，而UACC - 462显示有多个7号染色体拷贝。使用放射性标记的EGF受体cDNA探针进行的杂交研究未能在任何细胞系中证明DNA序列扩增，但证实了这些细胞中存在EGF受体mRNA，其比例与EGF受体数量大致相当。我们的结果表明，人胰腺癌中EGF受体表达增强可能与7号染色体的结构或数量改变有关。
