Anaya-Cubero Elena, Cortés Adriana, Lachén-Montes Mercedes, Cartas-Cejudo Paz, Fernández-Irigoyen Joaquín, Santamaría Enrique
Clinical Neuroproteomics Unit, Proteomics Platform, Navarrabiomed, Hospital Universitario de Navarra (HUN), Universidad Pública de Navarra (UPNA), IdiSNA, Pamplona, Spain.
Methods Mol Biol. 2025;2914:77-87. doi: 10.1007/978-1-0716-4462-1_7.
Cerebrospinal fluid (CSF) is a valuable source of biomarkers for neurological diseases due to its proximity to the central nervous system. Phosphorylation, a common post-translational modification, plays crucial roles in cellular signaling and is dysregulated in various neurological disorders.Analysis of the CSF phosphoproteome using titanium dioxide (TiO) enrichment coupled with high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique unveils a diverse array of phosphorylated proteins and their corresponding phosphorylation sites, shedding light on the intricate regulatory networks within CSF.The combination of TiO enrichment and high-resolution LC-MS/MS offers a robust and sensitive approach to studying the phosphoproteome, providing valuable insights into cellular signaling dynamics and disease mechanisms.
脑脊液（CSF）因其与中枢神经系统的接近性，是神经疾病生物标志物的宝贵来源。磷酸化是一种常见的翻译后修饰，在细胞信号传导中起关键作用，并且在各种神经疾病中失调。使用二氧化钛（TiO）富集结合高分辨率液相色谱-串联质谱（LC-MS/MS）技术对脑脊液磷酸化蛋白质组进行分析，揭示了多种磷酸化蛋白质及其相应的磷酸化位点，为脑脊液内复杂的调控网络提供了线索。TiO富集和高分辨率LC-MS/MS的结合为研究磷酸化蛋白质组提供了一种强大而灵敏的方法，为细胞信号传导动力学和疾病机制提供了有价值的见解。
