Cheng Lin, Wang Yanglu, Guan Jingyang, Deng Hongkui
MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences and MOE Engineering Research Center of Regenerative Medicine, School of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.
State Key Laboratory of Natural and Biomimetic Drugs, Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing, China.
Trends Biochem Sci. 2025 Jun;50(6):520-531. doi: 10.1016/j.tibs.2025.03.004. Epub 2025 Mar 31.
Pluripotent stem cells hold great promise as an unlimited resource for regenerative medicine due to their capacity to self-renew and differentiate into various cell types. Chemical reprogramming using small molecules precisely regulates cell signaling pathways and epigenetic states, providing a novel approach for generating human pluripotent stem cells. Since its successful establishment in 2022, human chemical reprogramming has rapidly achieved significant progress, demonstrating its significant potential in regenerative medicine. Mechanistic analyses have revealed distinct molecular pathways and regulatory mechanisms unique to chemical reprogramming, differing from traditional transcription-factor-driven methods. In this review we highlight recent advancements in our understanding of the mechanisms of human chemical reprogramming, with the goal of enhancing insights into the principles of cell fate control and advancing regenerative medicine.
多能干细胞作为再生医学的无限资源具有巨大潜力，因为它们能够自我更新并分化为各种细胞类型。使用小分子进行化学重编程可精确调节细胞信号通路和表观遗传状态，为生成人类多能干细胞提供了一种新方法。自2022年成功建立以来，人类化学重编程迅速取得了重大进展，显示出其在再生医学中的巨大潜力。机制分析揭示了化学重编程特有的不同分子途径和调控机制，这与传统的转录因子驱动方法不同。在这篇综述中，我们重点介绍了我们对人类化学重编程机制理解的最新进展，目的是加深对细胞命运控制原理的认识并推动再生医学的发展。
