Wang Pan, Yin Denghua, Jiang Min, Xie Lingli, Liu Jie, Chen Yukuan, Wang Xinyue, Shao Yan, Liu Kai
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
Sci Data. 2025 Apr 1;12(1):554. doi: 10.1038/s41597-025-04890-4.
Pseudorasbora elongata is a unique small fish species endemic to China, distinguished by its striking body coloration resembling a "Chinese ink brush." Due to environmental changes and anthropogenic factors, its wild populations have declined, and it has been listed multiple times as an endangered species. However, the absence of a chromosomal-level reference for P. elongata has hindered our understanding of its population genetics and conservation biology. To address this gap, we present a chromosome-level genome assembly of P. elongata, generated using PacBio HiFi reads, Oxford Nanopore Technologies, and Hi-C data. We get a genome size of 1.4 Gb with a contig N50 of 34.4 Mb and a scafold N50 of 53.7 Mb. Telomeric sequences were identified at the ends of 42 telomeres across 25 chromosomes. Notably, we observed a high degree of collinearity between our assembly and the Pseudorasbora parva genome. This study provides valuable insights into the genetics, genomics, and evolutionary history of P. elongata, offering a foundation for future research and enabling the development of genetic conservation strategies.
长麦穗鱼是中国特有的一种独特小型鱼类,其显著的体色类似“中国毛笔”,以此为特征。由于环境变化和人为因素,其野生种群数量已经减少,并且多次被列为濒危物种。然而,缺乏长麦穗鱼的染色体水平参考基因组阻碍了我们对其种群遗传学和保护生物学的理解。为了填补这一空白,我们展示了一个使用PacBio HiFi reads、牛津纳米孔技术和Hi-C数据生成的长麦穗鱼染色体水平基因组组装。我们获得了一个大小为1.4 Gb的基因组,其重叠群N50为34.4 Mb,支架N50为53.7 Mb。在25条染色体的42个端粒末端鉴定出了端粒序列。值得注意的是,我们观察到我们的组装与麦穗鱼基因组之间存在高度的共线性。这项研究为长麦穗鱼的遗传学、基因组学和进化历史提供了有价值的见解,为未来的研究奠定了基础,并有助于制定遗传保护策略。