Loh T T, Chan R Y, Fong W F
Cell Biol Int Rep. 1985 May;9(5):495-500. doi: 10.1016/0309-1651(85)90158-4.
An increase in extracellular spermine concentration brought about a progressive rise in intralysosomal pH in rabbit reticulocytes. Since intracellular release of iron from transferrin is believed to involve the protonation of the iron-transferrin complex, the rise in intralysomal pH could account for the inhibitory effect of spermine on iron uptake. The inhibition could be reversed if spermine was removed by washing. As a result of spermine treatment, more acid-labile N-terminal monoferric transferrin and less apotransferrin were released from the cell. These results are consistant with the protonation theory of iron release.
细胞外精胺浓度的增加导致兔网织红细胞溶酶体内pH值逐渐升高。由于人们认为转铁蛋白的铁从细胞内释放涉及铁 - 转铁蛋白复合物的质子化，溶酶体内pH值的升高可以解释精胺对铁摄取的抑制作用。如果通过洗涤去除精胺，这种抑制作用可以逆转。经过精胺处理后，更多酸不稳定的N端单铁转铁蛋白和更少的脱铁转铁蛋白从细胞中释放出来。这些结果与铁释放的质子化理论一致。
