兔网织红细胞在体外对与双铁转铁蛋白结合的铁的优先利用。
Preferential utilization in vitro of iron bound to diferric transferrin by rabbit reticulocytes.
作者信息
Zapolski E J, Princiotto J V
出版信息
Biochem J. 1977 Aug 15;166(2):175-9. doi: 10.1042/bj1660175.
59Fe uptake by rabbit reticulocytes from human transferrin-bound iron was studied by using transferrin solutions (35, 50, 65, 80 and 100% saturated with iron) whose only common characteristic was their content of diferric transferrin. During the early incubation period, 59Fe uptake from each preparation by reticulocytes was identical despite wide variations in amounts of total transferrin, total iron, monoferric transferrin and apotransferrin in solution. During the later phase of incubation, rate of uptake declined and was proportional to each solution's monoferric transferrin content. Uptake was also studied in a comparative experiment which used two identical, partially saturated transferrin preparations, one uniformly 59Fe-labelled and the other tracer-labelled with [59Fe]diferric transferrin. In both experiments, iron uptake by reticulocytes corresponded to utilization of a ferric ion from diferric transferrin before utilization of iron from monoferric transferrin.
通过使用仅有的共同特征是其三价铁转铁蛋白含量的转铁蛋白溶液（铁饱和度分别为35%、50%、65%、80%和100%），研究了兔网织红细胞从与人类转铁蛋白结合的铁中摄取⁵⁹Fe的情况。在孵育早期，尽管溶液中转铁蛋白总量、总铁量、单铁转铁蛋白和脱铁转铁蛋白的量存在很大差异，但网织红细胞从每种制剂中摄取⁵⁹Fe的情况是相同的。在孵育后期，摄取速率下降，且与每种溶液中单铁转铁蛋白的含量成正比。在一项对比实验中也研究了摄取情况，该实验使用了两种相同的、部分饱和的转铁蛋白制剂，一种均匀地用⁵⁹Fe标记，另一种用[⁵⁹Fe]三价铁转铁蛋白进行示踪标记。在这两个实验中，网织红细胞摄取铁的过程都表现为在利用单铁转铁蛋白中的铁之前，先利用三价铁转铁蛋白中的一个铁离子。
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