Yardim Şahika, Dursun Murat, Sözmen İlayda, Aydin Abdurrahman Fatih, Kadioğlu Ateş, Uslu Ünal, Demirci Elif Kervancioğlu
Istanbul Faculty of Medicine, Department of Histology and Embryology, Istanbul University, Istanbul, Turkey.
Faculty of Medicine, Department of Histology and Embryology, Istanbul Medeniyet University, Istanbul, Turkey.
Int J Urol. 2025 Jul;32(7):890-899. doi: 10.1111/iju.70059. Epub 2025 Apr 1.
Testicular torsion is a urological emergency with a risk of resulting in loss of testicular function due to ischemia/reperfusion injury (I/R) injury caused by oxidative stress. Ceratonia siliqua is known to decrease oxidative stress in urological pathologies. We investigated the protective effects of C. siliqua against I/R injury.
Wistar rats (n = 28) were divided into control, i.p. C. siliqua injected, I/R injury group, and C. siliqua injected each day for a week after I/R groups. They were morphologically evaluated with light and electron microscopy, and Caspase-3, Hypoxia-inducible factor 1 alpha (HIF-1), CD68, Superoxide dismutase (SOD), and Catalase antibodies were marked. Oxidative stress markers of Glutathione (GSH), malondialdehyde (MDA), reactive oxygen species (ROS), advanced oxidation protein products (AOPP), and ferric reducing antioxidant power (FRAP) from tissue and levels of testosterone, Anti-Müllerian hormone (AMH) and inhibin-B from blood samples were examined.
C.siliqua treatment preserved tissue integrity (p < 0.0001) and Leydig cells and decreased apoptotic (p = 0.0390) and necrotic changes when compared with the I/R group. The I/R group presented mitochondrial damage, intercellular edema, and vacuolization. Oxidative stress was less with treatment, but hormone levels were unchanged.
C.siliqua preserved the testicular tissue against I/R-induced damage. The decreased apoptosis may have been caused by the significant oxidative stress protection properties of C.siliqua. One week of treatment with C.siliqua protected Leydig cell morphology but did not change hormone parameters in the rat model. The therapeutic effect of C.siliqua is promising for clinical use in testis torsion cases.
睾丸扭转是一种泌尿外科急症，因氧化应激导致的缺血/再灌注损伤（I/R）有导致睾丸功能丧失的风险。已知长角豆可降低泌尿外科疾病中的氧化应激。我们研究了长角豆对I/R损伤的保护作用。
将28只Wistar大鼠分为对照组、腹腔注射长角豆组、I/R损伤组以及I/R后每天注射长角豆持续一周的组。通过光学和电子显微镜对其进行形态学评估，并标记半胱天冬酶-3、缺氧诱导因子1α（HIF-1）、CD68、超氧化物歧化酶（SOD）和过氧化氢酶抗体。检测组织中谷胱甘肽（GSH）、丙二醛（MDA）、活性氧（ROS）、晚期氧化蛋白产物（AOPP）和铁还原抗氧化能力（FRAP）的氧化应激标志物以及血样中睾酮、抗苗勒管激素（AMH）和抑制素B的水平。
与I/R组相比，长角豆治疗可保持组织完整性（p < 0.0001）和睾丸间质细胞，并减少凋亡（p = 0.0390）和坏死变化。I/R组出现线粒体损伤、细胞间水肿和空泡化。治疗后氧化应激减轻，但激素水平未改变。
长角豆可保护睾丸组织免受I/R诱导的损伤。凋亡减少可能是由于长角豆具有显著的氧化应激保护特性。在大鼠模型中，一周的长角豆治疗可保护睾丸间质细胞形态，但未改变激素参数。长角豆的治疗效果有望用于睾丸扭转病例的临床治疗。
