Le Nu Anh Thu, Kubo Rena, Borjigin Liushiqi, Ibi Takayuki, Sasaki Shinji, Kunieda Tetsuo
Faculty of Veterinary Medicine Okayama University of Science Imabari, Ehime, Japan.
Faculty of Animal Science and Veterinary Medicine University of Agriculture and Forestry, Hue University, Hue, Vietnam.
Anim Sci J. 2025 Jan-Dec;96(1):e70055. doi: 10.1111/asj.70055.
Mutations in the bovine myostatin (MSTN) gene have been identified as the causative factor for the double-muscling phenotype in several European cattle breeds, including Belgian Blue, Piedmontese, and Shorthorn. In Japan, following the Meiji Restoration, several European breeds, including Shorthorn, Brown Swiss, Devon, Simmental, and Ayrshire, were introduced and crossbred with native cattle to develop modern Japanese beef cattle breeds, such as Japanese Black cattle. Historical records regarding the breeding of Japanese Black cattle indicate that the double-muscling phenotype, referred to as "Butajiri," occasionally appeared in Japanese Black cattle population. These historical observations suggest the potential presence of MSTN gene mutation in the Japanese Black cattle population. The aim of this study was, therefore, to investigate the presence of MSTN gene mutation in the current Japanese Black cattle population. Through screening 400 reproductive females, we identified one cow carrying an 11-bp deletion in the MSTN gene. While further investigation of the animals in the pedigree of this cow could not reveal any living animals with this mutation, this is the first report demonstrating the presence of the MSTN mutation in the Japanese Black cattle population.
牛肌肉生长抑制素（MSTN）基因的突变已被确定为包括比利时蓝牛、皮埃蒙特牛和短角牛在内的几个欧洲牛品种双肌表型的致病因素。在日本，明治维新之后，包括短角牛、瑞士褐牛、德文牛、西门塔尔牛和艾尔夏牛在内的几个欧洲品种被引入，并与本地牛杂交，以培育现代日本肉牛品种，如日本黑牛。关于日本黑牛育种的历史记录表明，双肌表型，即所谓的“肉牛尻”，偶尔会出现在日本黑牛群体中。这些历史观察结果表明，日本黑牛群体中可能存在MSTN基因突变。因此，本研究的目的是调查当前日本黑牛群体中MSTN基因突变的存在情况。通过对400头繁殖母牛进行筛查，我们鉴定出一头母牛的MSTN基因存在11个碱基对的缺失。虽然对这头母牛家系中的动物进行进一步调查未能发现任何携带这种突变的活体动物，但这是首次报道在日本黑牛群体中存在MSTN突变。
