Mulya Harahap Raja Iqbal, Kurniati Intanri, Suraya Nida, Rostini Tiene, Ropii Bejo, Bethasari Maulidwina
Department of Clinical Pathology, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia.
Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia.
Int J Anal Chem. 2025 Mar 25;2025:8853568. doi: 10.1155/ianc/8853568. eCollection 2025.
Magnesium is a cation that plays as an important cofactor in various enzymatic reactions. It is the fourth most abundant cation in the body after sodium, potassium, and calcium. There are various magnesium measurement methods available such as spectrophotometry, atomic absorption spectrophotometry, and inductively coupled plasma-optical emission spectrophotometry. These measurement methods have various advantages and disadvantages in measuring magnesium levels in serum. This study aimed to compare the magnesium measurement results by using three different methods. A total of 221 samples were examined for magnesium levels using spectrophotometry, atomic absorption spectrophotometry, and inductively coupled plasma-optical emission spectrophotometry methods. The results were then grouped into hypomagnesemia, noromagnesemia, and hypermagnesemia according to normal values. The mean and standard deviation were calculated and compared across three different methods. The mean and standard deviation of serum magnesium ion levels measured using spectrophotometry, atomic absorption spectrophotometry, and inductively coupled plasma-optical emission spectrophotometry methods were 1.84 ± 0.43, 1.86 ± 0.43, and 1.85 ± 0.43 (mg/dL), respectively. There were no significant differences ( value > 0.05) in serum magnesium levels using spectrophotometry, atomic absorption spectrophotometry, and inductively coupled plasma-optical emission spectrophotometry measurement methods, indicating similar reliability among the methods.
镁是一种阳离子，在各种酶促反应中作为重要的辅助因子发挥作用。它是体内继钠、钾和钙之后含量第四丰富的阳离子。有多种镁的测量方法，如分光光度法、原子吸收分光光度法和电感耦合等离子体发射光谱法。这些测量方法在测量血清镁水平方面各有优缺点。本研究旨在比较使用三种不同方法测量镁的结果。总共221个样本分别采用分光光度法、原子吸收分光光度法和电感耦合等离子体发射光谱法检测镁水平。然后根据正常值将结果分为低镁血症、正常镁血症和高镁血症。计算三种不同方法的均值和标准差并进行比较。采用分光光度法、原子吸收分光光度法和电感耦合等离子体发射光谱法测量的血清镁离子水平的均值和标准差分别为1.84±0.43、1.86±0.43和1.85±0.43（mg/dL）。使用分光光度法、原子吸收分光光度法和电感耦合等离子体发射光谱法测量血清镁水平时无显著差异（P值>0.05），表明这些方法具有相似的可靠性。
