Malkin A, Reviczky M, Kellen J A
Clin Biochem. 1985 Apr;18(2):75-7. doi: 10.1016/s0009-9120(85)80084-9.
Whole extracts of normal human liver contain hCG-like material as determined by radioimmunoassay using antibodies to the beta subunit of the hormone. However, the extracts are biologically inactive, when analyzed by the in vitro rat Leydig cell assay for steroidogenesis. When subjected to Concanavalin-A-Sepharose chromatography, the radioimmunoassayable material was entirely lectin-bound. After elution with methyl-alpha-D-glucopyranoside, the lectin-bound fractions now displayed biological activity.
使用针对该激素β亚基的抗体，通过放射免疫测定法测定，正常人肝脏的全提取物含有类似人绒毛膜促性腺激素（hCG）的物质。然而，当通过体外大鼠睾丸间质细胞类固醇生成测定法进行分析时，这些提取物没有生物学活性。当进行伴刀豆球蛋白A-琼脂糖凝胶柱层析时，可通过放射免疫测定的物质完全与凝集素结合。用甲基-α-D-吡喃葡萄糖苷洗脱后，与凝集素结合的部分现在显示出生物学活性。
