Bogdanova Olga, Krause Katrin, Pietschmann Sebastian, Kothe Erika
Institute of Microbiology, Microbial Communication, Friedrich Schiller University Jena, Neugasse 25, 07743, Jena, Germany.
Environ Sci Pollut Res Int. 2025 Apr;32(17):10786-10799. doi: 10.1007/s11356-025-36330-6. Epub 2025 Apr 2.
The impact of soil and tree species on fungal and bacterial communities was investigated in a former uranium mining area with field and pot studies of the mycorrhizospheres of birch (Betula pendula), oak (Quercus robur), and pine (Pinus sylvestris). At the initial stages of succession re-created in the pot experiment, tree-species-specific microbial communities were detected. The pot microbiomes showed lower diversity and evenness of fungi and bacteria as compared to field-grown trees. In the natural field setting, the fungal community both in bulk and rhizosphere soil consisted of mainly Thelephoraceae, Inocybaceae and Russulaceae. They contributed with Leotiaceae and Herpotrichiellaceae to 52-85% of overall abundances, showing the soil hyphae impact of ectomycorrhiza in the tree stand. The fungal communities and their distribution patterns reflected host tree specificity and successional stage of the ectomycorrhizosphere. In the bacterial community, the most abundant bacterial classes were Alphaproteobacteria, Acidobacteria, Ktedonobacteria, Bacteroidia, Gammaproteobacteria, and Phycisphaerae representing about 59-80% of all bacterial sequences. The bacterial communities correlated with soil chemical parameters, particularly the content of toxic metals, total nitrogen and C/N ratio. This study allowed to identify drivers for microbial community composition, which might be helpful to develop afforestation strategies in post-mining landscapes.
通过对桦树（垂枝桦）、橡树（夏栎）和松树（欧洲赤松）菌根际进行田间和盆栽研究，调查了土壤和树种对真菌和细菌群落的影响。在盆栽实验重建演替的初始阶段，检测到了树种特异性的微生物群落。与田间生长的树木相比，盆栽微生物群落中真菌和细菌的多样性和均匀度较低。在自然田间环境中，块状土壤和根际土壤中的真菌群落主要由革菌科、丝膜菌科和红菇科组成。它们与肉座菌科和钉孢菌科共同构成了总丰度的52-85%，显示了外生菌根在林分中的土壤菌丝影响。真菌群落及其分布模式反映了宿主树的特异性和外生菌根际的演替阶段。在细菌群落中，最丰富的细菌类群是α-变形菌纲、酸杆菌纲、生金菌纲、拟杆菌纲、γ-变形菌纲和浮霉菌纲，约占所有细菌序列的59-80%。细菌群落与土壤化学参数相关，特别是有毒金属含量、总氮和碳氮比。这项研究有助于确定微生物群落组成的驱动因素，这可能有助于制定采矿后景观的造林策略。
