Quadri S K, Norman R L, Spies H G
Endocrinology. 1977 Feb;100(2):325-30. doi: 10.1210/endo-100-2-325.
Selected areas in the medial basal (MBH) and rostral (RH) hypothalamus and in the amygdala (AMYG) of long-term ovariectomized rhesus monkeys were electrically stimulated for 30 min through permanently implanted bilateral stainless steel electrodes. Stimulation of an area in the MBH extending from the dorsal part of the ventromedial nucleus through the arcurate nucleus to the upper median eminence resulted in a 200 to 400% increase within 5 min in 8 monkeys. In one monkey the elevated serum prolactin levels persisted after termination of stimulation and in 2 monkeys prolactin remained unchanged during the 30-min stimulation but increased after stimulation was discontinued. Stimulation of the paraventricular-dorsomedial nuclear area in one monkey had no effect on prolactin release. Prolactin responses to stimulation in the RH varied. In 2 monkeys the electrode tips extended into the optic chiasm but part of the uninsulated tips remained in contact with the RH; only one of these monkeys released prolactin in response to stimulation. In 4 monkeys the electrode tips were located in the suprachiasmatic-anterior hypothalamus area. Serum prolactin increased by 200 to 300% in response to stimulation in 2 of these monkeys but increased only slightly in the remaining 2 monkeys. Prolactin responses to stimulation of the AMYG varied with the location of the electrodes. Stimulation in the corticomedial region produced no change in serum prolactin but stimulation in the basal or basolateral area produced marked elevations. An increase in circulating levels of estradiol-17beta (E2) to 100 pg/ml by SC implantation of E2 capsules 72 h before stimulation had no significant effect on basal prolactin levels, but markedly enhanced the prolactin release induced by stimulation in both the MBH and RH. Sham-stimulation did not affect serum prolactin. We conclude that prolactin release in rhesus monkeys can be triggered by electrical stimulation of selected hypothalamic and amygdaloid areas and that stimulation-induced prolactin release in the RH and MBH can be enhanced by E2 pretreatment.
通过永久性植入的双侧不锈钢电极，对长期卵巢切除的恒河猴的内侧基底（MBH）和 Rostral（RH）下丘脑以及杏仁核（AMYG）的选定区域进行了 30 分钟的电刺激。刺激 MBH 中从腹内侧核背侧部分延伸至弓状核再到上正中隆起的区域，8 只猴子在 5 分钟内血清催乳素水平升高了 200%至 400%。在 1 只猴子中，刺激终止后血清催乳素水平持续升高，在 2 只猴子中，催乳素在 30 分钟刺激期间保持不变，但在刺激停止后升高。刺激 1 只猴子的室旁 - 背内侧核区域对催乳素释放没有影响。催乳素对 RH 刺激的反应各不相同。在 2 只猴子中，电极尖端延伸至视交叉，但部分未绝缘的尖端仍与 RH 接触；其中只有 1 只猴子在受到刺激时释放催乳素。在 4 只猴子中，电极尖端位于视交叉上 - 下丘脑前部区域。其中 2 只猴子在受到刺激时血清催乳素升高了 200%至 300%，但其余 2 只猴子仅略有升高。催乳素对 AMYG 刺激的反应因电极位置而异。刺激皮质内侧区域血清催乳素无变化，但刺激基底或基底外侧区域则显著升高。在刺激前 72 小时通过皮下植入 E2 胶囊使雌二醇 - 17β（E2）循环水平升高至 100 pg/ml，对基础催乳素水平无显著影响，但显著增强了 MBH 和 RH 中刺激诱导的催乳素释放。假刺激不影响血清催乳素。我们得出结论，恒河猴的催乳素释放可通过电刺激选定的下丘脑和杏仁核区域触发，并且 E2 预处理可增强 RH 和 MBH 中刺激诱导的催乳素释放。
