一种用于研究血清免疫球蛋白对小肠结肠炎耶尔森菌感染反应的动物模型。
An animal model to study serum immunoglobulin response to infection by Yersinia enterocolitica.
作者信息
Kono D H, Ogasawara M, Yu D T
出版信息
Clin Exp Rheumatol. 1985 Apr-Jun;3(2):117-21.
Using an Elisa method, we tested whether the serum immunoglobulin response of CBA/H mice, orally infected by a serotype 03 strain of Yersinia enterocolitica was of sufficient magnitude to serve as an experimental model. We found that the dose of bacteria administered was important. At a dose of 10(10)-10(12) bacteria per mouse, easily detectable IgM, IgG and IgA antibody titers could be obtained. At 10(8) bacteria per mouse, although diarrhea occurred and Yersinia was shed into the stool, the antibody titers were minimal.
我们采用酶联免疫吸附测定（ELISA）方法，检测经小肠结肠炎耶尔森氏菌03血清型菌株口服感染的CBA/H小鼠血清免疫球蛋白反应的强度是否足以作为一个实验模型。我们发现，所给予的细菌剂量很重要。每只小鼠给予10¹⁰ - 10¹²个细菌的剂量时，可轻松检测到IgM、IgG和IgA抗体滴度。每只小鼠给予10⁸个细菌时，尽管出现腹泻且耶尔森氏菌排至粪便中，但抗体滴度最低。
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