Hoefler Katharina, Sukop Ulrich, Scheler Stefan, Reiter Elisabeth, Bender Denisse, Jekle Mario, Schoenlechner Regine, D'Amico Stefano
AGES - Austrian Agency for Health and Food Safety, Institute for Animal Nutrition and Feed, Spargelfeldstraße 191, 1220 Vienna, Austria.
BOKU - University, Department of Biotechnology and Food Science, Muthgasse 18, 1190 Vienna, Austria.
J Agric Food Chem. 2025 Apr 16;73(15):9309-9319. doi: 10.1021/acs.jafc.5c02445. Epub 2025 Apr 2.
Arabinoxylans (AXs) are dietary fibers in monocotyledon cell walls that benefit digestive health and enhance food functionality. Despite their importance, no standardized method exists for AX quantification in gluten-free (GF) cereals. This study investigated the effect of hydrolysis parameters for AX quantification in GF cereals (maize, rice, oat) with varying AX content and nutritional profiles to address matrix effects. The effects of trifluoroacetic acid (TFA) concentration (0.25-4 M), temperature (90-127 °C), and time (1-5 h) on hydrolysis efficiency were examined, whereby temperature showed, in contrast to acid concentration and time, a pronounced influence. The design of experiment (DoE) model predicted 2 M TFA, 2.4 h, and 103 °C as the optimal conditions for maximizing AX yield without detectable monosaccharide decomposition for all varieties. This was experimentally confirmed with a deviation of less than 10%. An extensive validation confirmed the method's accuracy and reliability for this unified method.
阿拉伯木聚糖（AXs）是单子叶植物细胞壁中的膳食纤维，对消化健康有益，并能增强食品功能。尽管它们很重要，但目前尚无用于无麸质（GF）谷物中AX定量的标准化方法。本研究调查了水解参数对不同AX含量和营养成分的GF谷物（玉米、大米、燕麦）中AX定量的影响，以解决基质效应问题。研究了三氟乙酸（TFA）浓度（0.25 - 4 M）、温度（90 - 127 °C）和时间（1 - 5 h）对水解效率的影响，结果表明，与酸浓度和时间相比，温度对水解效率有显著影响。实验设计（DoE）模型预测，2 M TFA、2.4 h和103 °C是所有品种AX产量最大化且无明显单糖分解的最佳条件。实验证实了这一点，偏差小于10%。广泛的验证证实了该统一方法的准确性和可靠性。
