Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
Int J Biol Macromol. 2020 Dec 1;164:2284-2292. doi: 10.1016/j.ijbiomac.2020.08.113. Epub 2020 Aug 15.
Microalgal biopolymers are studied mainly in terms of physico-chemical characterization, biological effects as well as possible biotechnological applications. Due to the significant antitussive, bronchodilator, anti-inflammatory and immunomodulatory effects of the previously isolated crude extracellular polysaccharide (EPS) produced by the cyanobacterium Nostoc sp., the purified biopolymer and its oligosaccharides, obtained after partial acid hydrolysis, were subjected to an in-depth NMR structural study. Analyses of the data obtained by chemical methods and NMR showed that the EPS backbone is composed of the repeating unit [→4)-β-D-Xylp-(1 → 4)-β-D-Glcp-(1 → 4)-α-L-Arap-(1 → 3)-β-D-Manp-(1→], in which about 60% of glucose units are substituted at C6 by uronic acids, in particular by the unusual unsaturated 3-O-lactyl-4-deoxy-α-erythro-hex-4-enopyranuronic acid, and to a lesser extent by β-D-glucuronic acid and 3-O-lactyl-β-D-glucuronic acid. These findings, structural features and identified biological effects, suggest the potential use of this biopolymer in the medical-pharmaceutical field.
微藻生物聚合物主要从物理化学特性、生物效应以及可能的生物技术应用等方面进行研究。由于先前从蓝细菌 Nostoc sp. 中分离得到的粗细胞外多糖 (EPS) 具有显著的镇咳、支气管扩张、抗炎和免疫调节作用，因此对其纯生物聚合物及其经部分酸水解得到的寡糖进行了深入的 NMR 结构研究。通过化学方法和 NMR 分析得到的数据表明，EPS 主链由重复单元 [→4)-β-D-Xylp-(1 → 4)-β-D-Glcp-(1 → 4)-α-L-Arap-(1 → 3)-β-D-Manp-(1→] 组成，其中约 60%的葡萄糖单元在 C6 位被糖醛酸取代，特别是由不常见的不饱和 3-O-乳酰基-4-去氧-α-赤式-己-4-烯吡喃糖醛酸，以及较少的β-D-葡萄糖醛酸和 3-O-乳酰基-β-D-葡萄糖醛酸。这些发现、结构特征和鉴定的生物效应表明，该生物聚合物在医疗-制药领域具有潜在的应用价值。
