Laboratory of Food Chemistry, Wageningen University , P.O. Box 17, 6700 AA Wageningen, The Netherlands.
J Agric Food Chem. 2015 Dec 23;63(50):10822-30. doi: 10.1021/acs.jafc.5b04419. Epub 2015 Dec 15.
Cell wall material from whole oat grains was sequentially extracted to study the structural characteristics of individual arabinoxylan (AX) populations. Araf was singly substituted at both O-3 (mainly) and O-2 positions of Xylp, and no disubstitution of Xylp with Araf residues was found in oat AXs. Both highly substituted and sparsely substituted segments were found in AXs in Ba(OH)2 extracts, whereas AXs in 1 and 6 M NaOH extracts were rarely branched and easily aggregated. Both O-2-linked GlcA and 4-O-MeGlcA residues were present in oat AXs. A series of AX oligomers with galactose as a substituent was detected for the first time in oats. The present study suggested that the distribution of Araf was contiguous in oat AXs, different from the homogeneous distribution of Araf in wheat and barley AXs, which might result in different fermentation patterns in humans and animals.
采用分步提取法从整粒燕麦中提取细胞壁物质，以研究各阿拉伯木聚糖（AX）级分的结构特征。燕麦 AX 中的阿魏酰基仅在木糖基的 O-3 位（主要）和 O-2 位发生单取代，未发现木糖基上的阿魏酰基双取代。在 Ba(OH)2 提取物中发现了高度取代和稀疏取代的 AX 片段，而在 1 M 和 6 M NaOH 提取物中的 AX 分支很少且易于聚集。燕麦 AX 中存在 O-2 连接的葡萄糖醛酸和 4-O-甲基葡萄糖醛酸残基。首次在燕麦中检测到一系列以半乳糖为取代基的 AX 低聚物。本研究表明，燕麦 AX 中的阿魏酸呈连续分布，与小麦和大麦 AX 中阿魏酸的均匀分布不同，这可能导致人和动物的发酵模式不同。
