Schenkelaars Nicole, Wekema Lieske, Faas Marijke M, Steegers-Theunissen Régine P M, Schoenmakers Sam
Department of Obstetrics and Gynaecology, Erasmus Medical Center, Rotterdam, The Netherlands.
Department of Pathology and Medical Biology, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands.
PLoS One. 2025 Apr 2;20(4):e0319618. doi: 10.1371/journal.pone.0319618. eCollection 2025.
Preconceptional and maternal obesity are well-known risk factors for pregnancy and fetal complications including gestational diabetes, hypertensive disorders, and macrosomia. Maternal obesity is associated with offspring obesity and increased healthcare costs. To disrupt the cycle of obesity, we aim to investigate the impact of the composition of the maternal microbiota (bacteria and viruses) throughout preconception and pregnancy and the associations with the immune responses and one-carbon metabolism (1-CM) as an underlying mechanism in the pathophysiology of increased adverse pregnancy outcomes in maternal obesity.
The PROMOTE study is a subcohort of the Rotterdam Periconceptional Cohort, a hospital-based observational cohort study. We will include 70 women per BMI group: ≥ 30 kg/m2 or 18.5-25 kg/m2, at different time points in each group: 10 preconceptional, 50 in the first trimester (with longitudinal follow-up during pregnancy, delivery and postpartum) and 10 in the third trimester of pregnancy. Which makes a total of 140 inclusions. Vaginal and rectal bacteriome, virome, and blood samples are collected. In the third trimester inclusions, only faecal samples are collected. Microbiota samples will be analysed using 16S rRNA sequencing. Bacteriome and virome profiles are compared between the BMI subgroups, associations with general immune responses and 1-CM markers will be shown.
ClinicalTrials.gov (NCT05754645).
孕前肥胖和孕期肥胖是众所周知的妊娠及胎儿并发症风险因素，包括妊娠期糖尿病、高血压疾病和巨大儿。孕期肥胖与子代肥胖及医疗费用增加有关。为打破肥胖循环，我们旨在研究孕前及孕期母体微生物群（细菌和病毒）组成的影响，以及其与免疫反应和一碳代谢（1-CM）的关联，将其作为孕期肥胖中不良妊娠结局增加的病理生理学潜在机制。
PROMOTE研究是鹿特丹孕前队列的一个亚队列，这是一项基于医院的观察性队列研究。我们将在每个BMI组中纳入70名女性：BMI≥30kg/m²或BMI为18.5-25kg/m²，在每组的不同时间点纳入：10名孕前女性、50名孕早期女性（在孕期、分娩期和产后进行纵向随访）以及10名孕晚期女性。总共纳入140名女性。收集阴道和直肠细菌群落、病毒群落及血液样本。对于孕晚期纳入的女性，仅收集粪便样本。微生物群样本将使用16S rRNA测序进行分析。比较BMI亚组之间的细菌群落和病毒群落谱，展示其与一般免疫反应和1-CM标志物的关联。
ClinicalTrials.gov（NCT05754645）。
