Li Shenglin, Gu Qibing, Li Benrun, Abi Kehamo, Yang Falong
College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China.
College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China.
Vet J. 2025 Jun;311:106344. doi: 10.1016/j.tvjl.2025.106344. Epub 2025 Mar 31.
Pasteurella multocida is one of the most important pathogens that infect goats, causing serious economic losses in the goat breeding industry. To understand the biological characteristics of P. multocida from goats, a comprehensive characterization of bacteria isolated from 342 nasal swabs and 8 lung tissue samples from goat farms in Sichuan, China, was performed. A total of 34 isolates were assigned to one capsular type, D, and one lipopolysaccharide (LPS) genotype, L3, indicating that the D: L3 was the predominant serotype in goat farms. In the 34 isolates, multiple virulence-related genes were identified, with a detection rate of 100 % (34/34) for the genes ompA, ompH, oma87, exbB, and exbD. It is noteworthy that the prevalence of the toxA gene, which encodes the P. multocida toxin (PMT), was found to be 85.2 % (29/34). Furthermore, antimicrobial susceptibility testing indicated a high prevalence of multidrug resistance, with resistance rates of 41.1 % for ampicillin, 38.2 % for tetracycline, and 32.3 % for kanamycin. Overall, this study provides a foundational understanding of the epidemiology and antimicrobial resistance of P. multocida in goats, offering insights for future prevention and control measures.
多杀性巴氏杆菌是感染山羊的最重要病原体之一，给山羊养殖业造成严重经济损失。为了解来自山羊的多杀性巴氏杆菌的生物学特性，对从中国四川山羊养殖场采集的342份鼻拭子和8份肺组织样本中分离出的细菌进行了全面鉴定。共34株分离菌被鉴定为一种荚膜型（D型）和一种脂多糖（LPS）基因型（L3型），表明D:L3是山羊养殖场的主要血清型。在这34株分离菌中，鉴定出多个与毒力相关的基因，ompA、ompH、oma87、exbB和exbD基因的检出率为100%（34/34）。值得注意的是，编码多杀性巴氏杆菌毒素（PMT）的toxA基因的流行率为85.2%（29/34）。此外，抗菌药物敏感性试验表明多重耐药性的发生率很高，氨苄西林的耐药率为41.1%，四环素为38.2%，卡那霉素为32.3%。总体而言，本研究为多杀性巴氏杆菌在山羊中的流行病学和抗菌药物耐药性提供了基础认识，为未来的预防和控制措施提供了见解。
