Liu Fuyan, Du Lin, Li Tingting, Liu Bo, Guo Jianyang, Zhang Guifen, Zhang Yibo, Liu Wanxue, Pan Yan, Zhang Ying, Wang Hailing, Li Ruiying, Song Weining, Wan Fanghao
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, 712100, Shaanxi, China.
OmicsGang Biotechnology Corporation, 18 Yuan Street, Beijing, 101318, China.
Sci Data. 2025 Apr 2;12(1):560. doi: 10.1038/s41597-025-04637-1.
Crofton weed (Ageratina adenophora), a significant invasive species, extensively disrupts ecosystem stability, leading to considerable economic losses. However, genetic insights into its invasive mechanisms have been limited by a lack of genomic data. In this study, we present the successful de novo assembly of the triploid genome of A. adenophora, leveraging long-read PacBio Sequel, optical mapping, and Hi-C sequencing. Our assembly resolved into a haplotype-resolved genome comprising 51 chromosomes, with a total size of ~3.82 Gb and a scaffold N50 of 70.8 Mb. BUSCO analysis confirmed the completeness of 97.71% of genes. Genome annotation revealed 3.16 Gb (76.44%) of repetitive sequences and predicted 123,134 protein-coding genes, with 99.03% functionally annotated. The high-quality reference genome will provide valuable genomic resources for future studies on the evolutionary dynamics and invasive adaptations of A. adenophora.
紫茎泽兰（Ageratina adenophora）是一种重要的入侵物种，它广泛破坏生态系统稳定性，导致巨大的经济损失。然而，由于缺乏基因组数据，对其入侵机制的遗传学认识一直有限。在本研究中，我们利用长读长PacBio Sequel、光学图谱和Hi-C测序技术，成功地对紫茎泽兰的三倍体基因组进行了从头组装。我们的组装结果解析为一个包含51条染色体的单倍型解析基因组，总大小约为3.82 Gb，支架N50为70.8 Mb。BUSCO分析证实97.71%的基因是完整的。基因组注释显示有3.16 Gb（76.44%）的重复序列，并预测了123,134个蛋白质编码基因，其中99.03%的基因有功能注释。高质量的参考基因组将为未来关于紫茎泽兰进化动态和入侵适应性的研究提供有价值的基因组资源。
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