Zhang Jianbo, Zhao Wenji, Tang Xiaohui, Jia Guofu, Li Daxu, Xia Chongjing, Bai Shiqie
Sichuan Academy of Grassland Sciences, Chengdu, China.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, China.
Sci Data. 2025 Apr 14;12(1):620. doi: 10.1038/s41597-025-04962-5.
Rhodiola kirilowii, a perennial medical herb native to China, is highly valued for its detoxification and anti-swelling properties, as well as its role as an adaptogen, making it an intriguing subject for understanding its medicinal potential and molecular biochemistry. In this study, we generated a high-quality chromosome-level reference genome of R. kirilowii achieved through a combination of Illumina short-read sequencing, PacBio long-read sequencing, and Hi-C sequencing techniques. The final assembly spans 1.92 Gb, including 40 homoeologous chromosomes and one sex chromosome, with a scaffold NG50 of 46.03 Mb, and a BUSCO completeness of 98.9%. Additionally, we annotated a total of 1.23 Gb of repetitive sequences, encompassing 63.88% of the entire genome, and identified 122,035 protein-coding genes. Each sub-genome achieved similar completeness and continuity. This high-quality reference genome provides critical insights into the genetic underpinnings of R. kirilowii's pharmacological properties, facilitating comparative genomics and the enhancement of its medicinal applications.
红景天是一种原产于中国的多年生药草，因其解毒消肿特性以及作为适应原的作用而备受重视，这使其成为了解其药用潜力和分子生物化学的一个有趣课题。在本研究中，我们通过结合Illumina短读长测序、PacBio长读长测序和Hi-C测序技术，生成了高质量的红景天染色体水平参考基因组。最终组装基因组跨度为1.92 Gb，包括40条同源染色体和1条性染色体，支架N50为46.03 Mb，BUSCO完整性为98.9%。此外，我们共注释了1.23 Gb的重复序列，占整个基因组的63.88%，并鉴定出122,035个蛋白质编码基因。每个亚基因组都具有相似的完整性和连续性。这个高质量的参考基因组为红景天药理特性的遗传基础提供了关键见解，有助于比较基因组学研究并促进其药用应用的提升。
