粗茎秋海棠（秋海棠科）的染色体水平基因组组装

A chromosomal-level genome assembly of Begonia fimbristipula (Begoniaceae).

作者信息

Xiao Tian-Wen, Wang Zheng-Feng, Yan Hai-Fei

机构信息

Key Laboratory of National Forestry and Grassland Administration on Plant Conservation and Utilization in Southern China, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.

South China National Botanical Garden, Guangzhou, 510650, China.

出版信息

Sci Data. 2025 Mar 12;12(1):429. doi: 10.1038/s41597-025-04768-5.

DOI:10.1038/s41597-025-04768-5

PMID:40074751

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11904028/

Abstract

Begonia fimbristipula Hance (Begoniaceae) is a valuable medicinal herb that is classified as a protected species in Guangdong Province, China. In this study, we present a chromosome-level genome assembly of B. fimbristipula, aiming to facilitate its conservation and utilization. The genome was assembled using a combination of Oxford Nanopore long-read data and Illumina short-read data. The assembled genome size of B. fimbristipula is 462.11 Mb, with a scaffold N50 of 38.22 Mb. A total of 91.96% (424.94 Mb) of the sequences were anchored to 11 pseudochromosomes using Hi-C technology. The genome assembly exhibits a BUSCO completeness of 90.3% and an LTR Assembly Index (LAI) of 17.73. Genome annotation revealed 25,563 protein-coding genes and 274 tRNA genes. The high-quality chromosome-level assembly and annotation provide valuable insights into the genomic characteristics of B. fimbristipula, thereby offering essential resources for its conservation and economic utilization.

摘要

紫背天葵（秋海棠科）是一种珍贵的药用植物，在中国广东省被列为保护物种。在本研究中，我们展示了紫背天葵的染色体水平基因组组装，旨在促进其保护和利用。该基因组是使用牛津纳米孔长读长数据和Illumina短读长数据相结合的方法进行组装的。紫背天葵组装后的基因组大小为462.11 Mb，支架N50为38.22 Mb。利用Hi-C技术，共91.96%（424.94 Mb）的序列被锚定到11条假染色体上。该基因组组装的BUSCO完整性为90.3%，LTR组装指数（LAI）为17.73。基因组注释显示有25,563个蛋白质编码基因和274个tRNA基因。高质量的染色体水平组装和注释为紫背天葵的基因组特征提供了有价值的见解，从而为其保护和经济利用提供了重要资源。