Autilio Chiara, Touqui Lhousseine, Foligno Silvia, Arroyo Raquel, Kingma Paul S, Alonso Alejandro A, Perez-Gil Jesus, De Luca Daniele
Department of Biochemistry and Molecular Biology, Faculty of Biology, and Research Institut Hospital 12 de Octubre, Complutense University, Madrid, Spain.
Centre de Recherche Saint-Antoine (CRSA)-INSERM U938, Sorbonne Université, Paris, France.
J Perinatol. 2025 Apr 2. doi: 10.1038/s41372-025-02285-2.
To study the effect of hypothermia on surfactant proteins, anti-inflammatory and pro-fibrotic mediators DESIGN: Prospective, pragmatic study enrolling asphyxiated neonates without lung injury. Surfactant proteins (SP), Club cell secretory protein (CC-10), tumor growth factor-β1 (TGF-β1), human fibroblast growth factor-2 (hFGF) and anti-inflammatory interleukins (IL) were measured in broncho-alveolar lavage fluids obtained before, during and after hypothermia.
Twelve neonates were studied. SP-A, -B, -C, -D and CC-10 levels were similar before, during and after hypothermia. IL-10, IL-13, IL-14 were inconsistently detected only in five out of twelve patients and not in all timepoints; TGF-β1 was always undetectable. hFGF decreased during hypothermia (pre = 92 [52-193.6], during = 19.4 [0-42.6], post = 31.8 [0-83] ng/mL, p = 0.026) with levels before hypothermia being significantly higher than those obtained during and after the treatment (post hoc p = 0.025).
In cooled neonates without any lung disorder, hypothermia is associated with lower hFGF but not with any changes in surfactant proteins or anti-inflammatory molecules.
研究低温对表面活性蛋白、抗炎和促纤维化介质的影响。
一项前瞻性、实用性研究，纳入无肺损伤的窒息新生儿。在低温治疗前、治疗期间和治疗后采集的支气管肺泡灌洗液中检测表面活性蛋白（SP）、克拉拉细胞分泌蛋白（CC-10）、肿瘤生长因子-β1（TGF-β1）、人成纤维细胞生长因子-2（hFGF）和抗炎白细胞介素（IL）。
研究了12例新生儿。低温治疗前、治疗期间和治疗后，SP-A、-B、-C、-D和CC-10水平相似。IL-10、IL-13、IL-14仅在12例患者中的5例中检测结果不一致，且并非在所有时间点均能检测到；TGF-β1始终未检测到。低温治疗期间hFGF水平降低（治疗前=92[52-193.6]，治疗期间=19.4[0-42.6]，治疗后=31.8[0-83]ng/mL，p=0.026），治疗前水平显著高于治疗期间和治疗后水平（事后检验p=0.025）。
在无任何肺部疾病的低温治疗新生儿中，低温与较低的hFGF相关，但与表面活性蛋白或抗炎分子的任何变化无关。
