Thermo Fisher Scientific, Carlsbad, CA 92008, United States.
Thermo Fisher Scientific, Carlsbad, CA 92008, United States.
Methods. 2019 Apr 1;158:27-32. doi: 10.1016/j.ymeth.2019.01.018. Epub 2019 Feb 8.
The ability to simultaneously measure multiple secreted proteins and the corresponding gene expression levels from a single sample is valuable for comprehensive analysis. Bottlenecks to traditional immunoassays and gene expression assays include large sample consumption, time consuming experimental procedures, and complex data analysis.
Here, we demonstrate two high-throughput assays measuring both messenger RNA (mRNA) expression and proteins in a single sample run on a Luminex platform. Human peripheral blood mononuclear cells (hPBMCs) were treated with lipopolysaccharide (LPS) and harvested at 24 and 72 h. Samples were assayed with the ProcartaPlex Human Immune Monitoring 65-plex Panel for protein and corresponding mRNA targets on a QuantiGene Human 80-plex Panel.
Multiplexing ProcartaPlex and QuantiGene Plex assays provide a broad survey of protein levels and gene expression networks.
能够从单个样本中同时测量多种分泌蛋白及其相应的基因表达水平，对于综合分析非常有价值。传统免疫测定和基因表达测定的瓶颈包括大量样本消耗、耗时的实验程序和复杂的数据分析。
在这里，我们展示了两种高通量测定方法，可在 Luminex 平台上同时测量单个样本中的信使 RNA（mRNA）表达和蛋白质。用人外周血单核细胞（hPBMC）用脂多糖（LPS）处理，并在 24 和 72 小时收获。样品用 ProcartaPlex 人类免疫监测 65 plex 面板进行蛋白质和相应的 mRNA 靶标检测，并在 QuantiGene 人类 80 plex 面板上进行检测。
ProcartaPlex 和 QuantiGene Plex 测定的多重性提供了蛋白质水平和基因表达网络的广泛调查。
