Libotte Manon, Zuccheri Giampaolo
Department of Pharmacy and Biotechnology, Alma Mater Studiorum Università di Bologna, Bologna, Italy.
Interdepartmental Center for Industrial Research on Life Science and Health Technologies, Alma Mater Studiorum Università di Bologna, Bologna, Italy.
Methods Mol Biol. 2025;2901:1-12. doi: 10.1007/978-1-0716-4394-5_1.
The nature of DNA allows building highly predictable nanostructures that have been applied in a wide range of areas like chemical sensing, material science, molecular data storage, and nanoelectronics. In the field of nanomedicine, DNA nanostructures proved a useful tool for diagnostic and therapeutic purposes. This chapter provides the protocols for the synthesis of self-assembled DNA nanostructures such as the DNA tetrahedron and the characterization of its assembly. Moreover, it also gives the protocols for the characterization of the internalization in mammalian cells both qualitatively and quantitatively.
DNA的特性使其能够构建高度可预测的纳米结构，这些纳米结构已应用于广泛的领域，如化学传感、材料科学、分子数据存储和纳米电子学。在纳米医学领域，DNA纳米结构被证明是用于诊断和治疗目的的有用工具。本章提供了自组装DNA纳米结构（如DNA四面体）的合成方案及其组装表征。此外，还给出了定性和定量表征其在哺乳动物细胞内化的方案。
