通过杂交链式反应检测线粒体ATP的方法
Method for Detecting Mitochondrial ATP by Hybridization Chain Reaction.
作者信息
Luo Lei, Yang Xiaohai, Wang Kemin
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, China.
出版信息
Methods Mol Biol. 2025;2901:159-165. doi: 10.1007/978-1-0716-4394-5_12.
Adenosine triphosphate (ATP) plays a central role in energy transduction and signaling in living cells. For mitochondrial ATP detection, the appropriate probes should include the abilities to enter target cells noninvasively, target mitochondria, and then respond to the ATP reliably. Here, we provide a detailed protocol for imaging mitochondrial ATP in living cells exploiting the hybridization chain reaction (HCR).
三磷酸腺苷（ATP）在活细胞的能量转导和信号传导中起着核心作用。对于线粒体ATP检测，合适的探针应具备无创进入靶细胞、靶向线粒体并可靠地响应ATP的能力。在此，我们提供了一份利用杂交链式反应（HCR）对活细胞中线粒体ATP进行成像的详细方案。
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