School of Pharmaceutical Sciences , Sun Yat-sen University , Guangzhou 510006 , China.
College of Materials and Energy , South China Agriculatural University , Guangzhou 510642 , China.
ACS Appl Mater Interfaces. 2019 Apr 3;11(13):12244-12252. doi: 10.1021/acsami.8b21998. Epub 2019 Mar 19.
Synergy represents a natural approach for high-efficiency recognition in biological systems. Inspired by the recognition mechanism of viral infection of mammalian cells, here we develop heterovalency magnetic DNA nanoclaws with octopus arms morphology for synergetic cell capture. We demonstrated that the rigid-flexible DNA nanoclaws can load multiple antibodies (Abs) targeting different epitopes for enhanced capture of cancer cells, especially significantly increasing the capture efficiency of MDA-MB-231 cells up to 82.3 ± 7.1%. We also employed DNA nanoclaws with the combined use of multiple Abs to capture circulating tumor cells from clinical samples with high efficiency and specificity. We expect that the DNA nanoclaws not only could play a key role in liquid biopsy, but also could be expanded, with more applications benefiting from their modularity and programmability to modify various functionalities in future.
协同作用代表了生物系统中高效识别的一种自然方法。受哺乳动物细胞病毒感染识别机制的启发，我们开发了具有章鱼臂形态的杂价磁性 DNA 纳米爪，用于协同细胞捕获。我们证明，刚性-柔性 DNA 纳米爪可以加载多个针对不同表位的抗体 (Abs)，以增强对癌细胞的捕获，特别是显著提高了 MDA-MB-231 细胞的捕获效率，达到 82.3 ± 7.1%。我们还使用具有多种 Abs 联合使用的 DNA 纳米爪，从临床样本中高效、特异地捕获循环肿瘤细胞。我们期望 DNA 纳米爪不仅可以在液体活检中发挥关键作用，而且还可以扩展，通过它们的模块化和可编程性，在未来修改各种功能，从而获得更多的应用。
