使用双参数流式细胞术对大鼠睾丸细胞进行DNA分析和分选。

DNA analysis and sorting of rat testis cells using two-parameter flow cytometry.

作者信息

van Kroonenburgh M J, Beck J L, Scholtz J W, Hacker-Klom U, Herman C J

出版信息

Cytometry. 1985 Jul;6(4):321-6. doi: 10.1002/cyto.990060408.

Abstract

By use of two-parameter flow cytometry of rat testis cell suspensions stained with mithramycin for DNA (the peak amplitude of the fluorescence signal versus total fluorescence intensity integrated over time), eight cell compartments could be distinguished without pre-enrichment of the samples. Cells in these compartments were identified by sorting and subsequent microscopic examination.

摘要

通过使用用光辉霉素对大鼠睾丸细胞悬液进行DNA染色的双参数流式细胞术（荧光信号的峰值幅度与随时间积分的总荧光强度），无需对样品进行预富集即可区分八个细胞区室。通过分选和随后的显微镜检查来鉴定这些区室中的细胞。