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多个近红外发光二极管（700、850和980纳米）连续波光生物调节对MG63成骨细胞线粒体呼吸和基因表达的影响

Effects of Multiple Near-Infrared LEDs (700, 850, and 980 nm) CW-PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts.

作者信息

Sleep Simone, Hryciw Deanne H, Walsh Laurence J, Ranjit Eliza, Tomy Nifty, Arany Praveen R, George Roy

机构信息

School of Medicine and Dentistry, Griffith University, Southport, Australia.

School of Environment and Science, Griffith University, Nathan, Australia.

出版信息

J Biophotonics. 2025 Aug;18(8):e70015. doi: 10.1002/jbio.70015. Epub 2025 Apr 3.

DOI:10.1002/jbio.70015
PMID:40177989
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12318645/
Abstract

BACKGROUND AND AIM: This study evaluated mitochondrial and osteogenic activity in MG-63 pre-osteoblastic cells after photobiomodulation (PBM) using multiple near-infrared LED sources (Nuralyte) emitting wavelengths from 700 to 1100 nm. MATERIALS AND METHODS: MG-63 cells were irradiated daily for 3, 5, or 7 days with energy densities of 5.3 J/cm (30 s, optimal dose) and 10.6 J/cm (60 s, high dose). Mitochondrial function was assessed using the XF Seahorse analyzer, and gene expression of osteogenic markers was analyzed. RESULTS: Maximal mitochondrial oxygen consumption rate (OCR) significantly decreased at the optimal dose but increased at the high dose (p < 0.001) in 5-day irradiated cultures. Upregulation of osteogenic markers (OCN, OPN, BMP-2, COL-1, RUNX2) occurred after 3-5 consecutive days of irradiation, with greater activation at the optimal dose. CONCLUSION: MG-63 cells respond to PBM using MNI-LEDs (700, 850, 980 nm) by modulating mitochondrial respiration and boosting bone-related gene expression in a dose- and time-dependent manner.

摘要

背景与目的：本研究评估了使用发射波长为700至1100nm的多个近红外发光二极管光源（Nuralyte）进行光生物调节（PBM）后，MG-63前成骨细胞中的线粒体和成骨活性。 材料与方法：MG-63细胞分别以5.3J/cm²（30秒，最佳剂量）和10.6J/cm²（60秒，高剂量）的能量密度，每日照射3、5或7天。使用XF Seahorse分析仪评估线粒体功能，并分析成骨标志物的基因表达。 结果：在照射5天的培养物中，最佳剂量下最大线粒体氧消耗率（OCR）显著降低，但高剂量下则升高（p<0.001）。连续照射3至5天后，成骨标志物（骨钙素、骨桥蛋白、骨形态发生蛋白-2、I型胶原蛋白、RUNX2）上调，最佳剂量下激活程度更高。 结论：MG-63细胞通过使用MNI-LEDs（700、850、980nm）进行PBM，以剂量和时间依赖性方式调节线粒体呼吸并促进骨相关基因表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b9b/12318645/dfde4b7769b2/JBIO-18-e70015-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b9b/12318645/f68a04fb505f/JBIO-18-e70015-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b9b/12318645/63b3a52f731b/JBIO-18-e70015-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b9b/12318645/06ca569ddb5b/JBIO-18-e70015-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b9b/12318645/dfde4b7769b2/JBIO-18-e70015-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b9b/12318645/f68a04fb505f/JBIO-18-e70015-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b9b/12318645/63b3a52f731b/JBIO-18-e70015-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b9b/12318645/06ca569ddb5b/JBIO-18-e70015-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b9b/12318645/dfde4b7769b2/JBIO-18-e70015-g001.jpg

相似文献

[1]
Effects of Multiple Near-Infrared LEDs (700, 850, and 980 nm) CW-PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts.

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本文引用的文献

[1]
Assessment of the onset of analgesia and length of analgesia following the use of PBM with different wavelengths: a clinical study.

Lasers Med Sci. 2024-9-19

[2]
Laser light: Illuminating the path to enhanced periodontal care.

Photodiagnosis Photodyn Ther. 2024-4

[3]
The Effects of Photobiomodulation Over Fibroblasts and Extracellular Matrix.

Photobiomodul Photomed Laser Surg. 2024-2

[4]
Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer.

Bioengineering (Basel). 2024-1-13

[5]
The Role of Low-Level Laser Therapy in Bone Healing: Systematic Review.

Int J Mol Sci. 2023-4-12

[6]
Transcriptome analysis of the effects of polarized photobiomodulation on human dermal fibroblasts.

J Photochem Photobiol B. 2023-5

[7]
Runx2 regulates chromatin accessibility to direct the osteoblast program at neonatal stages.

Cell Rep. 2022-9-6

[8]
Effectiveness of Photobiomodulation Therapy on Human Bone Healing in Dentistry: A Systematic Review.

Photobiomodul Photomed Laser Surg. 2022-7

[9]
Bioenergetics of photobiomodulated osteoblast mitochondrial cells derived from human pulp stem cells: systematic review.

Lasers Med Sci. 2022-4

[10]
Comparison of Single and Multiple Low-Level Laser Applications After Rapid Palatal Expansion on Bone Regeneration in Rats.

J Lasers Med Sci. 2020

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