Du Xian, Zhang Chunxiao, Peng Biyu
College of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, Sichuan, China.
National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu, 610065, Sichuan, China.
Bioprocess Biosyst Eng. 2025 Jun;48(6):981-992. doi: 10.1007/s00449-025-03157-x. Epub 2025 Apr 3.
A method based on fluorescently labeled enzyme proteins was established to visualize the absorption properties of lipase at the oil-water interface, and it can be used for the effective observation of the distribution characteristics of lipase at the oil-water interface. The optimal conditions for observation include the following: oil content of 10-20% (wt%), concentration of fluorescently labelled enzyme protein of 0.25 mg/mL, reaction temperature of 25-30 °C, emulsion dispersion and stirring time of 10 min, and emulsion resting time of 30-120 s. Based on this method, a preliminary analysis of the effects of oil and lipase species on the distribution characteristics of lipase at the oil-water interface was performed. The results reveal that differences in the distributions of lipase at the oil-water interface of various fats and oils had a certain degree of correspondence with their specificity and that the distribution characteristics of the lipases on the surface of olive oil enabled effective catalytic hydrolysis to a certain extent. This method is a more objective guide for the development of lipase application technology in the fields of tanning, fur making, glue making, detergents, and sewage treatment and so on.
建立了一种基于荧光标记酶蛋白的方法来可视化脂肪酶在油水界面的吸附特性，可用于有效观察脂肪酶在油水界面的分布特征。观察的最佳条件如下：油含量为10 - 20%（重量），荧光标记酶蛋白浓度为0.25 mg/mL，反应温度为25 - 30°C，乳液分散和搅拌时间为10分钟，乳液静置时间为30 - 120秒。基于该方法，对油和脂肪酶种类对脂肪酶在油水界面分布特征的影响进行了初步分析。结果表明，不同油脂在油水界面处脂肪酶分布的差异与其特异性有一定程度的对应关系，且橄榄油表面脂肪酶的分布特征在一定程度上能够实现有效的催化水解。该方法为制革、制裘、制胶、洗涤剂及污水处理等领域脂肪酶应用技术的开发提供了更客观的指导。
