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脂肪酶在两相系统和微乳液界面的催化反应。

Lipase-catalyzed reactions at interfaces of two-phase systems and microemulsions.

机构信息

Nestlé Research Center, 1000 Lausanne 26, Switzerland.

出版信息

Appl Biochem Biotechnol. 2009 Sep;158(3):706-21. doi: 10.1007/s12010-008-8354-5. Epub 2008 Sep 16.

DOI:10.1007/s12010-008-8354-5
PMID:18795240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2847170/
Abstract

This work describes the influence of two polar lipids, Sn-1/3 and Sn-2 monopalmitin, on the activity of lipase in biphasic systems and in microemulsions. In previous communications, we have shown that Sn-2 monoglycerides can replace Sn-1,3 regiospecific lipases at the oil-water interface, causing a drastically reduced rate of lipolysis. We here demonstrate that even if the lipase is expelled from the interface, it can catalyze esterification of the Sn-2 monoglyceride with fatty acids in both macroscopic oil-water systems and in microemulsions, leading to formation of di- and triglycerides.

摘要

本工作描述了两种极性脂，Sn-1/3 和 Sn-2 单棕榈酸甘油酯，对脂肪酶在双相体系和微乳液中的活性的影响。在以前的交流中，我们已经表明，Sn-2 单甘油脂可以替代油-水界面处的 Sn-1,3 区域专一性脂肪酶，导致脂肪水解的速率大大降低。我们在此证明，即使脂肪酶从界面中被逐出，它也可以在宏观油-水体系和微乳液中催化脂肪酸与 Sn-2 单甘油脂的酯化反应，生成二酯和三酯。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/25d78e8eeddf/12010_2008_8354_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/1e98a7d7d8ff/12010_2008_8354_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/809b7ce53b3c/12010_2008_8354_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/a0e52bdc7918/12010_2008_8354_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/6a5e568dcda5/12010_2008_8354_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/f7de9ff9f7bf/12010_2008_8354_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/99a51df60876/12010_2008_8354_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/a6ca80312f72/12010_2008_8354_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/61096ab17555/12010_2008_8354_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/25d78e8eeddf/12010_2008_8354_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/1e98a7d7d8ff/12010_2008_8354_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/809b7ce53b3c/12010_2008_8354_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/a0e52bdc7918/12010_2008_8354_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/6a5e568dcda5/12010_2008_8354_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/f7de9ff9f7bf/12010_2008_8354_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/99a51df60876/12010_2008_8354_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/a6ca80312f72/12010_2008_8354_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/61096ab17555/12010_2008_8354_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab37/2847170/25d78e8eeddf/12010_2008_8354_Fig9_HTML.jpg

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本文引用的文献

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Influence of Surfactants on Lipase Fat Digestion in a Model Gastro-intestinal System.表面活性剂对模拟胃肠系统中脂肪酶消化脂肪的影响。
Food Biophys. 2008 Dec;3(4):370-381. doi: 10.1007/s11483-008-9091-6. Epub 2008 Jun 26.
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Lipases at interfaces: a review.界面处的脂肪酶：综述
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Esterification reactions catalyzed by Chromobacterium viscosum lipase in CTAB-based microemulsion systems.在基于十六烷基三甲基溴化铵的微乳液体系中，粘性色杆菌脂肪酶催化的酯化反应。
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Competition between lipases and monoglycerides at interfaces.脂肪酶与甘油单酯在界面处的竞争。
Langmuir. 2008 Jul 15;24(14):7400-7. doi: 10.1021/la800531y. Epub 2008 Jun 12.
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Adsorption of polar lipids at the water-oil interface.极性脂质在水-油界面的吸附。
Langmuir. 2008 Jun 3;24(11):5781-6. doi: 10.1021/la704043g. Epub 2008 May 3.
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