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经皮电刺激神经疗法可改善前交叉韧带损伤大鼠并调节肠道微生物群。

TENS improves CFL injury rat and regulates the intestinal microbiota.

作者信息

Miao Min, Ma Tong, Chen Ran, Geng Kuan, Shen Zhiqiang, Sun Yan

机构信息

Pharmaceutical College and Key Laboratory of Pharmacology for Natural Products of Yunnan Province, Kunming Medical University, Kunming, Yunnan, P.R. China.

The College of Modern Biomedical Industry, Kunming Medical University, Kunming, Yunnan, P.R. China.

出版信息

PLoS One. 2025 Apr 3;20(4):e0319592. doi: 10.1371/journal.pone.0319592. eCollection 2025.

DOI:10.1371/journal.pone.0319592
PMID:40179112
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11967936/
Abstract

AIM

This study revealed the mechanism of transcutaneous electrical nerve stimulation (TENS) for improving the calcaneofibular ligament (CFL) injury rats by regulating the intestinal microbiota.

METHODS

After 1, 2, and 3 weeks of TENS treatment, the improvement of CFL injury rats model and the expressions of IL-1β/NF-κB/IL-17 signaling pathway were measured. Then the intestinal microbiota was analyzed by 16S rDNA sequencing and its functions related to improve CFL injury rat were analyzed.

RESULTS

TENS could improved the athletic ability of CFL injury rats and reduced the expressions of IL-1β/NF-κB by regulating the IL-17 signaling pathway. By 16S rDNA sequencing analysis, the TENS treatment improved the intestinal dysbacteriosisof CFL injury rats and decrease pathogenic bacteria Ruminococcus and Dubosiella. The changed intestinal microbiota maybe relative with the ankle injury, whereas the increase in probiotics (Bacteroides and Lactobacillus) was relative with anti-inflammation.

CONCLUSION

TENS could down-regulate the expressions of IL-1β/NF-κB to improve CFL injury rat. TENS could change the intestinal microbiota of CFL rats and the changed bacteria whose function related to anti-inflammation could improve CFL rat. The intestinal microbiota could become a potential treatment for CFL injury.

摘要

目的

本研究揭示经皮电刺激神经（TENS）通过调节肠道微生物群改善跟腓韧带（CFL）损伤大鼠的机制。

方法

在TENS治疗1、2和3周后，测量CFL损伤大鼠模型的改善情况以及IL-1β/NF-κB/IL-17信号通路的表达。然后通过16S rDNA测序分析肠道微生物群，并分析其与改善CFL损伤大鼠相关的功能。

结果

TENS可通过调节IL-17信号通路提高CFL损伤大鼠的运动能力，并降低IL-1β/NF-κB的表达。通过16S rDNA测序分析，TENS治疗改善了CFL损伤大鼠的肠道菌群失调，并减少了病原菌瘤胃球菌和杜波氏菌。肠道微生物群的变化可能与踝关节损伤有关，而益生菌（拟杆菌和乳酸杆菌）的增加与抗炎有关。

结论

TENS可下调IL-1β/NF-κB的表达以改善CFL损伤大鼠。TENS可改变CFL大鼠的肠道微生物群，且其功能与抗炎相关的变化细菌可改善CFL大鼠。肠道微生物群可能成为CFL损伤的一种潜在治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/1833e1561e03/pone.0319592.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/3c038448c54f/pone.0319592.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/ea95f2e7b7b0/pone.0319592.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/483fad0e3683/pone.0319592.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/1d727daa3b64/pone.0319592.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/35c440535be8/pone.0319592.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/4e79053c3bdf/pone.0319592.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/1833e1561e03/pone.0319592.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/3c038448c54f/pone.0319592.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/ea95f2e7b7b0/pone.0319592.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/483fad0e3683/pone.0319592.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/1d727daa3b64/pone.0319592.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/35c440535be8/pone.0319592.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/4e79053c3bdf/pone.0319592.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1197/11967936/1833e1561e03/pone.0319592.g007.jpg

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