Hayat Rubina, Shah Syed Zakir Hussain, Fatima Mahroze, Hussain Syed Makhdoom, Ashraf Shahzad, Ali Wazir
Department of Zoology, University of Gujrat, Gujrat, Pakistan.
Department of Zoology, University of Gujrat, Gujrat, Pakistan.
J Trace Elem Med Biol. 2025 Jun;89:127639. doi: 10.1016/j.jtemb.2025.127639. Epub 2025 Mar 29.
This experiment determined the Mn requirement of Cirrhinus mrigala and evaluated its effects on growth indices and health status. Six experimental diets were prepared containing 0, 5, 10, 15, 20 and 25 mg/kg Mn from MnSO·HO and analyzed Mn contents were 1.48, 6.37, 11.24, 16.35, 20.8 and 26.15 mg/kg, respectively. A total of 270 C. mrigala juveniles (average initial weight 12.784 ± 0.613 g) were acclimatized and distributed to eighteen aquaria (70 L capacity and 15 fish/aquarium). Each experimental diet was fed in triplicate up to satiation for 60 days. After completion of trial, growth performance and feed intake increased with increasing Mn concentrations in diets up to 26.15 mg/kg. However, feed conversion ratio (FCR) decreased with increasing Mn concentration up to 16.35 mg/kg. Furthermore, whole-body proximate composition improved with increasing Mn concentrations up to 16.35 mg/kg. Mn content in vertebrae, whole body, muscles, kidney and scales significantly increased with increments in Mn concentrations in the diet, however, Mn absorption reached at saturation at 16.35 mg/kg. Hepatic alkaline phosphatase and antioxidant enzymes activities increased significantly with increasing Mn concentrations up to16.35 mg/kg in the diet, while opposite trend was observed in thiobarbituric acid reactive substances (TBARS) content. Based on weight gain%, FCR, Mn content in vertebrae and TBARS content, dietary Mn requirements of the C. mrigala juveniles were assessed to be 13.18, 16.35, 17.22 and 16.45 mg/kg, respectively. In conclusion, C. mrigala require 13.18-17.22 mg/kg Mn in diet for optimum growth performance and health status.
本实验确定了印度鲮对锰的需求量，并评估了其对生长指标和健康状况的影响。制备了六种实验饲料，分别含有0、5、10、15、20和25mg/kg由硫酸锰·水合物提供的锰，分析得到的锰含量分别为1.48、6.37、11.24、16.35、20.8和26.15mg/kg。总共270尾印度鲮幼鱼（平均初始体重12.784±0.613g）进行了驯化，并分配到18个水族箱（容量70L，每个水族箱15尾鱼）。每种实验饲料设三个重复组，投喂至饱足，持续60天。试验结束后，饲料中锰浓度增至26.15mg/kg时，生长性能和采食量随之增加。然而，饲料转化率（FCR）在锰浓度增至16.35mg/kg时下降。此外，饲料中锰浓度增至16.35mg/kg时，鱼体的常规成分得到改善。饲料中锰浓度增加时，椎骨、鱼体、肌肉、肾脏和鳞片中的锰含量显著增加，但锰的吸收在16.35mg/kg时达到饱和。饲料中锰浓度增至16.35mg/kg时，肝脏碱性磷酸酶和抗氧化酶活性显著增加，而硫代巴比妥酸反应物质（TBARS）含量则呈现相反趋势。根据增重率、FCR、椎骨中的锰含量和TBARS含量，评估印度鲮幼鱼的饲料锰需求量分别为13.18、16.35、17.22和16.45mg/kg。总之，印度鲮在饲料中需要13.18 - 17.22mg/kg的锰以实现最佳生长性能和健康状况。
