Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Department of Zoology, University of Gujrat, Gujrat, Pakistan.
PLoS One. 2024 Aug 12;19(8):e0308761. doi: 10.1371/journal.pone.0308761. eCollection 2024.
This study was conducted to investigate the effects of selenium nanoparticle (Se-NP) supplementation on the growth performance, carcass composition, antioxidant status, hepatic enzyme activities, and immunity of Cirrhinus mrigala. For this purpose, fish with an average initial weight of 7.44 ± 0.04 g were fed five experimental diets containing 0 (control), 0.25, 0.5, 1, and 2 mg kg-1 Se-NPs diets for 90 days. The analysed selenium (Se) contents of the diets were 0.35, 0.64, 0.92, 1.43, and 2.39 mg kg-1. Twenty five fish were randomly distributed in each of 5 aquarium (36 × 23.7 × 24.3 inches) in triplicate. The results showed that supplementation with Se up to 0.92 mg/kg significantly increased (p<0.05) weight gain, weight gain% (WG%), and specific growth rate (SGR) by 34%, 33%, and 16%, respectively, compared to the control diet. Dietary Se concentrations up to 0.92 mg/kg significantly increased the crude protein and crude fat and reduced (p<0.05) the moisture content as compared to the control group. Fish fed 0.92 mg kg-1 Se had significantly lower malondialdehyde (MDA) contents and higher activities of catalase, superoxide dismutase, and glutathione peroxidase in liver and serum as compared to other experimental diets. Moreover, a significant increase (p<0.05) in the level of serum immunoglobulin and lysozyme (LYZ) activity was recorded in fish fed 0.92 mg/kg Se diet. Moreover, the highest (p<0.05) values of aspartate transaminase (AST) and alanine transaminase (ALT) were recorded in fish fed 2.39 mg/kg Se level. However, serum alkaline phosphatase (ALP) activity remained unaffected by dietary treatment. Broken-line regression analysis indicated that 0.83 mg/kg Se is required for the optimum growth performance of C. mrigala.
本研究旨在探讨硒纳米颗粒（Se-NP）补充对圆口铜鱼生长性能、体组成、抗氧化状态、肝酶活性和免疫的影响。为此，将平均初始体重为 7.44±0.04 g 的鱼用五种实验饲料喂养 90 天，饲料中分别含有 0（对照）、0.25、0.5、1 和 2 mg/kg Se-NP。分析的饲料硒（Se）含量分别为 0.35、0.64、0.92、1.43 和 2.39 mg/kg。将 25 条鱼随机分配到 5 个水族箱（36×23.7×24.3 英寸）中，每个水族箱重复 3 次。结果表明，与对照组相比，添加至 0.92 mg/kg 的 Se 显著提高（p<0.05）增重率、增重率%（WG%）和特定生长率（SGR），分别提高了 34%、33%和 16%。饲料中 Se 浓度高达 0.92 mg/kg 时，与对照组相比，粗蛋白和粗脂肪显著增加（p<0.05），水分含量显著降低（p<0.05）。与其他实验饲料相比，摄食 0.92 mg kg-1 Se 的鱼肝脏和血清中的丙二醛（MDA）含量较低，过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶的活性较高。此外，摄食 0.92 mg/kg Se 饲料的鱼血清免疫球蛋白和溶菌酶（LYZ）活性显著升高（p<0.05）。此外，摄食 2.39 mg/kg Se 水平的鱼天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）活性最高（p<0.05）。然而，血清碱性磷酸酶（ALP）活性不受膳食处理的影响。折线回归分析表明，圆口铜鱼的最佳生长性能需要 0.83 mg/kg Se。
