沿屠宰线的零售鸭肉中同时携带fosA3和ESBL基因的多重耐药大肠杆菌的出现。
Emergence of multidrug resistant Escherichia coli coharboring fosA3 and ESBL genes from retail ducks along slaughter line.
作者信息
Chen Jialan, Kuang Ruyi, Liu Haotian, Yang Lei, Li Jiarui, Li Junlin, Xia Jing, Huang Yong, Cui Min, Zou Likou, Han Xinfeng
机构信息
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of China, Chengdu, China; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China.
出版信息
Int J Food Microbiol. 2025 May 2;435:111185. doi: 10.1016/j.ijfoodmicro.2025.111185. Epub 2025 Mar 29.
The excessive and indiscriminate use of antimicrobial agents in poultry production has increased antimicrobial resistance in E. coli, posing a significant threat to public health. This study investigated the prevalence of multidrug-resistant (MDR) E. coli co-harboring fosA3 and extended-spectrum β-lactamases (ESBLs) genes in large-scale retail duck slaughterhouses in Sichuan Province, China. The antimicrobial susceptibility to 9 categories and 16 types of antimicrobial agents was assessed by using the broth microdilution method. Phylogenetic grouping and pulsed-field gel electrophoresis (PFGE) were used to investigate the phylogenetic groups and genetic characteristics of the isolates. Moreover, whole-genome sequencing (WGS) was performed on 35 representative E. coli isolates. Among 1, 059 samples collected, 895 E. coli were isolated by MacConkey (MAC) agar and eosin methylene blue (EMB) agar and PCR identification, and 150 strains of MDR E. coli co-carrying fosA3 and ESBL genes were finally screened for further analysis. The results indicated that 141 isolates were resistant to fosfomycin and exhibited high resistance to β-lactam antibiotics. All isolates demonstrated MDR patterns, with 148 isolates resistant to six or more classes of antimicrobial agents. The majority (70/150, 46.67 %) belonged to phylogenetic group B1. Based on an 85 % similarity threshold, all isolates were categorized into 40 distinct PFGE types. Genomic analysis revealed 27 different serotypes and 19 sequence types (STs), with O8 (14.29 %, 5/35) and ST155 (22.86 %, 8/35) being the most common. The IncFIB (AP001918) was the most prevalent plasmid replicon type, identified in 74.29 % (26/35) of the isolates. In addition, 49 acquired antimicrobial resistance genes (ARGs) associated with resistance to 11 types of antimicrobial agents were identified and chromosomal mutation of p.S83L in gyrA gene (88.57 %, 31/35) was the most common. Furthermore, 110 virulence factors (VFs) were identified, with those related to iron uptake and storage, adhesion, secretion and endotoxin production being the most prevalent. This study underscored the importance of rational use of antimicrobial agents in poultry farming and provided critical insights into the distribution of ARGs and VFs among retail duck-derived MDR E. coli.
家禽生产中抗菌剂的过度和随意使用增加了大肠杆菌的耐药性，对公众健康构成重大威胁。本研究调查了中国四川省大型零售鸭屠宰场中同时携带fosA3和超广谱β-内酰胺酶（ESBLs）基因的多重耐药（MDR）大肠杆菌的流行情况。采用肉汤微量稀释法评估了9类16种抗菌剂的药敏性。利用系统发育分组和脉冲场凝胶电泳（PFGE）研究分离株的系统发育组和遗传特征。此外，对35株具有代表性的大肠杆菌分离株进行了全基因组测序（WGS）。在采集的1059份样本中，通过麦康凯（MAC）琼脂、伊红美蓝（EMB）琼脂和PCR鉴定分离出895株大肠杆菌，最终筛选出150株同时携带fosA3和ESBL基因的MDR大肠杆菌菌株进行进一步分析。结果表明，141株分离株对磷霉素耐药，并对β-内酰胺类抗生素表现出高度耐药性。所有分离株均呈现MDR模式，其中148株对6类或更多类抗菌剂耐药。大多数（70/150，46.67%）属于系统发育组B1。基于85%的相似性阈值，所有分离株被分为40种不同的PFGE型。基因组分析揭示了27种不同的血清型和19种序列型（STs），其中O8（14.29%，5/35）和ST155（22.86%，8/35）最为常见。IncFIB（AP001918）是最常见的质粒复制子类型，在74.29%（26/35）的分离株中被鉴定到。此外，鉴定出49个与11种抗菌剂耐药相关的获得性抗菌耐药基因（ARGs），gyrA基因中p.S83L的染色体突变最为常见（88.57%，31/35）。此外，鉴定出110个毒力因子（VFs），其中与铁摄取和储存、黏附、分泌及内毒素产生相关的毒力因子最为普遍。本研究强调了家禽养殖中合理使用抗菌剂的重要性，并为零售鸭源MDR大肠杆菌中ARGs和VFs的分布提供了关键见解。
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