Single molecular profile of proteins sensing by nanopore technology.

The characterization of biological macromolecules such as proteins and their interactions are crucial to understanding biological processes, disease diagnosis, and drug design. With the rapid development of proteomics, nanopore technology has emerged potentially as a single-molecule profile for huge amounts of peptides and proteins defined in the biological system, particularly for protein sequencing. This review focuses on recent advances in nanopore sensing of proteins and peptides, involving protein dynamic interactions, protein fingerprinting, and protein sequencing. These progresses will provide new perspectives to decipher the mechanisms of protein structure and function, and serve much more possible applications.