State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
IET Nanobiotechnol. 2023 May;17(3):257-268. doi: 10.1049/nbt2.12124. Epub 2023 Mar 16.
As biological macromolecules, proteins are involved in important cellular functions ranging from DNA replication and biosynthesis to metabolic signalling and environmental sensing. Protein sequencing can help understand the relationship between protein function and structure, and provide key information for disease diagnosis and new drug design. Nanopore sensors are a novel technology to achieve the goal of label-free and high-throughput protein sequencing. In recent years, nanopore-based biosensors have been widely used in the detection and analysis of biomolecules such as DNA, RNA, and proteins. At the same time, computer simulations can describe the transport of proteins through nanopores at the atomic level. This paper reviews the applications of nanopore sensors in protein sequencing over the past decade and the solutions to key problems from a computer simulation perspective, with the aim of pointing the way to the future of nanopore protein sequencing.
作为生物大分子，蛋白质参与了从 DNA 复制和生物合成到代谢信号传递和环境感应等重要的细胞功能。蛋白质测序有助于理解蛋白质功能和结构之间的关系，并为疾病诊断和新药设计提供关键信息。纳米孔传感器是实现无标记和高通量蛋白质测序目标的一项新技术。近年来，基于纳米孔的生物传感器已广泛应用于 DNA、RNA 和蛋白质等生物分子的检测和分析。同时，计算机模拟可以在原子水平上描述蛋白质通过纳米孔的传输。本文综述了过去十年中纳米孔传感器在蛋白质测序中的应用，以及从计算机模拟角度解决关键问题的方法，旨在为纳米孔蛋白质测序的未来指明方向。
