Wang Furong, Wang Xian, Xiao Yilei, Liu Ruixin, Li Xiaoben, Hu Jinpeng, Song Wei, Feng Kewei, Yuan Yahong, Yue Tianli
College of Food Science and Technology, Northwest University, Xi'an, Shaanxi 710069, China; Laboratory of Nutritional and Healthy Food-Individuation Manufacturing Engineering, Xi'an, Shaanxi 710069, China; Research Center of Food Safety Risk Assessment and Control, Xi'an, Shaanxi 710069, China.
College of Food Science and Technology, Northwest University, Xi'an, Shaanxi 710069, China; Laboratory of Nutritional and Healthy Food-Individuation Manufacturing Engineering, Xi'an, Shaanxi 710069, China; Research Center of Food Safety Risk Assessment and Control, Xi'an, Shaanxi 710069, China.
J Hazard Mater. 2025 Jul 15;492:138129. doi: 10.1016/j.jhazmat.2025.138129. Epub 2025 Apr 1.
Patulin (PAT) is a foodborne mycotoxin that causes intestinal injury. Selenium (Se)-enriched Kazachstania unispora (K. unispora) KU2 is a novel dietary Se carrier, and Se exerts important roles in intestinal homeostasis. Here, we examined the ameliorative effects of K. unispora KU2 and Se-enriched K. unispora KU2 against PAT-induced intestinal injury. Results indicated that both K. unispora KU2 and Se-enriched K. unispora KU2 alleviated PAT-induced inflammatory infiltration, disrupted gut microbiota, and associated metabolic imbalances, indicating the probiotic potential of this strain. Se-enriched K. unispora KU2 exhibited more pronounced remediation comparable to K. unispora KU2, revealing the promoting effect of Se. Furthermore, Se-enriched K. unispora KU2 restored intestinal Se homeostasis by upregulating SEPP1 levels to mitigate intestinal injury. Using pseudo germ-free mouse models, we confirmed that gut microbiota was required for the improvement in SEPP1 synthesis and intestinal transport mediated by Se-enriched K. unispora KU2. These findings elucidate a mechanism whereby the alleviation of PAT-induced intestinal injury by Se-enriched K. unispora KU2 is linked to upregulation of SEPP1 by the gut microbiota, suggesting its potential therapeutic relevance for intestinal diseases.
展青霉素（PAT）是一种食源性霉菌毒素，可导致肠道损伤。富硒单孢酵母（K. unispora）KU2是一种新型膳食硒载体，硒在肠道稳态中发挥重要作用。在此，我们研究了单孢酵母KU2和富硒单孢酵母KU2对PAT诱导的肠道损伤的改善作用。结果表明，单孢酵母KU2和富硒单孢酵母KU2均能减轻PAT诱导的炎症浸润、肠道微生物群紊乱及相关代谢失衡，表明该菌株具有益生菌潜力。与单孢酵母KU2相比，富硒单孢酵母KU2表现出更显著的修复作用，揭示了硒的促进作用。此外，富硒单孢酵母KU2通过上调SEPP1水平来恢复肠道硒稳态，以减轻肠道损伤。使用伪无菌小鼠模型，我们证实肠道微生物群是富硒单孢酵母KU2介导的SEPP1合成和肠道转运改善所必需的。这些发现阐明了一种机制，即富硒单孢酵母KU2减轻PAT诱导的肠道损伤与肠道微生物群上调SEPP1有关，表明其对肠道疾病具有潜在的治疗意义。
