Xu Zi-Jian, Xiao Dan, Chen Ke, Han Jin-Hui, Zhu Kong-Di, Liu Chuan-Ming, Wang Lian-Shun, Yu Zheng-Quan, Huang Jia-Qiang
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China.
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Poult Sci. 2025 Aug 13;104(11):105675. doi: 10.1016/j.psj.2025.105675.
Intestinal inflammation in chicks remains a prevalent health concern, highlighting the importance of developing effective alternatives to conventional antibiotics. This study aimed to investigate the effect of selenium-enriched Lactobacillus coryniformis H8 (Se-H8) in alleviating lipopolysaccharides (LPS)-induced intestinal inflammation in chicks and the related mechanisms. 252 1-day-old White Leghorn chicks (n = 6 cages/treatment, 6 chicks/cage) were randomly divided into seven groups (Control, LPS, H8, Se-H8, H8-NaSeO, NaSeO, and ENR). After 14 days of continuous gavage, a 3-day LPS intraperitoneal stress injection was performed. Serum, jejunum, and cecal faeces were collected for analysis of selenium levels, inflammatory factors, antioxidant status, gene expression, and microbial composition. Compared to the other treatment groups, Se-H8 exhibited the most pronounced alleviation of LPS-induced intestinal damage. This superior efficacy was reflected in significantly improved jejunal morphology, elevated serum selenium levels (P < 0.05), enhanced antioxidant capacity (increased GPX and SOD activities, decreased MDA levels; P < 0.05), and modulated inflammatory responses (reduced IL-1β and TNF-α, increased IL-10; P < 0.05). Furthermore, Se-H8 altered the expression of 23 selenoprotein genes in the jejunum and suppressed mRNA expression of key genes (TLR4, MyD88, NF-κB, IL-1β, TNF-α, and IL-10) in the TLR4/MyD88/NF-κB signaling pathway compared to the LPS group. Additionally, cecal microbiological analyses showed that Se-H8 increased the abundance of seven beneficial microorganisms, including Eubacterium_oxidoreducens_group, Eisenbergiella, Enorma, Catenibacillus, Merdibacter, Lachnospiraceae_FCS020_group, and GCA-900066575. In conclusion, Se-H8 alleviates LPS-induced jejunal injury by regulating antioxidant status, inflammation levels, selenoprotein genes expression, and gut microbial homeostasis. These findings highlight the potential of selenium-enriched microorganisms like Se-H8 as viable antibiotic alternatives for managing enteritis in poultry.
雏鸡肠道炎症仍然是一个普遍存在的健康问题，这凸显了开发替代传统抗生素的有效方法的重要性。本研究旨在探讨富硒棒状乳杆菌H8（Se-H8）对减轻脂多糖（LPS）诱导的雏鸡肠道炎症的作用及其相关机制。将252只1日龄白来航雏鸡（n = 6个笼子/处理组，6只雏鸡/笼子）随机分为七组（对照组、LPS组、H8组、Se-H8组、H8-NaSeO组、NaSeO组和恩诺沙星组）。连续灌胃14天后，进行为期3天的LPS腹腔应激注射。收集血清、空肠和盲肠粪便，用于分析硒水平、炎症因子、抗氧化状态、基因表达和微生物组成。与其他处理组相比，Se-H8对LPS诱导的肠道损伤的缓解最为明显。这种卓越的效果体现在空肠形态显著改善、血清硒水平升高（P < 0.05）、抗氧化能力增强（GPX和SOD活性增加，MDA水平降低；P < 0.05）以及炎症反应得到调节（IL-1β和TNF-α降低，IL-10增加；P < 0.05）。此外，与LPS组相比，Se-H8改变了空肠中23种硒蛋白基因的表达，并抑制了TLR4/MyD88/NF-κB信号通路中关键基因（TLR4、MyD88、NF-κB、IL-1β、TNF-α和IL-10）的mRNA表达。此外，盲肠微生物学分析表明，Se-H8增加了七种有益微生物的丰度，包括氧化还原梭菌属组、艾氏菌属、巨大杆菌属、链状芽孢杆菌属、梅迪杆菌属、毛螺菌科FCS020组和GCA-900066575。总之，Se-H8通过调节抗氧化状态、炎症水平、硒蛋白基因表达和肠道微生物稳态来减轻LPS诱导的空肠损伤。这些发现凸显了像Se-H8这样的富硒微生物作为管理家禽肠炎的可行抗生素替代品的潜力。
