Ferm V H, Hanlon D P
Environ Res. 1985 Aug;37(2):425-32. doi: 10.1016/0013-9351(85)90124-0.
We have examined the teratogenic and embryotoxic effects of constant-rate exposure of pregnant hamsters to arsenate by means of subcutaneous implants of osmotic minipumps. Different total exposure regimens were established by varying the duration of minipump implants and by varying the concentration of arsenate in the minipumps. Dams were killed on Day 13 of pregnancy, 5 days after the critical stage of organogenesis. Numbers of resorptions, dead fetuses, and living fetuses were obtained. Fetal weights, crown-rump lengths, and the incidence of malformations were recorded. Control animals were treated identically with minipumps containing demineralized water. The percentage of malformations per litter, a direct measure of teratogenesis, was dependent only upon the concentration of arsenate in the minipumps. The minimum teratogenic response was achieved with a dose of 70 mumol/kg dam/24 hr during the critical stages of organogenesis. The embryotoxic (fetotoxic) indicators, fetal weight and crown-rump length, decreased with increases in exposure time and with increased concentrations of arsenate. The resorption rate also depended directly upon duration of exposure and concentration of arsenate in the minipump.
我们通过皮下植入渗透微型泵，研究了妊娠仓鼠持续暴露于砷酸盐时的致畸和胚胎毒性作用。通过改变微型泵植入的持续时间以及微型泵中砷酸盐的浓度，建立了不同的总暴露方案。在器官发生关键阶段后的第5天，即妊娠第13天处死母鼠。记录吸收胎、死胎和活胎的数量。记录胎儿体重、顶臀长度和畸形发生率。对照动物用含有去离子水的微型泵进行相同处理。每窝畸形率是致畸作用的直接指标，仅取决于微型泵中砷酸盐的浓度。在器官发生关键阶段，剂量为70 μmol/kg母鼠/24小时时可产生最小致畸反应。胚胎毒性（胎儿毒性）指标，即胎儿体重和顶臀长度，随暴露时间的增加和砷酸盐浓度的升高而降低。吸收率也直接取决于暴露持续时间和微型泵中砷酸盐的浓度。
