Hoovler D W, Bernstein J J
Exp Neurol. 1985 Aug;89(2):337-47. doi: 10.1016/0014-4886(85)90094-9.
Morphological aspects of the implantation of 11-day-old fetal (E11) rat cortex into regenerating peripheral nerves of adult rats were analyzed during the first 3 weeks after transplantation. On day 11 of gestation, donor fetuses were removed from one uterine horn of pregnant Sprague-Dawley rats and pieces of cortex (1.0 X 0.5 mm) were dissected from them for transplantation. Fetuses and normally delivered pups from the other uterine horn were used as age-matched controls. In 45 adult male Sprague-Dawley rat hosts (200 to 300 g), the epineurium of the nerve to the biceps femoris muscle was crushed and incised, the perineurium minced, and E11 donor cortex was injected into the nerve. At 1 to 7 days postimplantation (DPI), undifferentiated neuroepithelial cells were dispersed or in oval neuroepithelial-like structures throughout the regenerating nerve. At 7 to 14 DPI, neurons and neuroglia were differentiating and interspersed among fascicles of regenerating host axons. At 14 to 21 DPI, aggregates of differentiated neurons surrounded by neuropil containing a rich synaptic bed were interspersed with regenerated host peripheral axons. Fetal cortical implants survived, increased in size, and differentiated throughout the postoperative period, matching their timed controls.
在移植后的前三周，对11日龄胎儿（E11）大鼠皮质植入成年大鼠再生周围神经的形态学方面进行了分析。在妊娠第11天，从怀孕的Sprague-Dawley大鼠的一个子宫角取出供体胎儿，并从其中切取皮质块（1.0×0.5毫米）用于移植。将来自另一个子宫角的胎儿和正常分娩的幼崽用作年龄匹配的对照。在45只成年雄性Sprague-Dawley大鼠宿主（200至300克）中，将股二头肌神经的神经外膜挤压并切开，将神经束膜切碎，并将E11供体皮质注入神经。在植入后1至7天（DPI），未分化的神经上皮细胞分散或呈椭圆形神经上皮样结构分布在整个再生神经中。在7至14 DPI时，神经元和神经胶质细胞正在分化并散布在再生宿主轴突束之间。在14至21 DPI时，由含有丰富突触床的神经毡包围的分化神经元聚集体与再生的宿主周围轴突交错分布。胎儿皮质植入物存活下来，大小增加，并在整个术后期间分化，与它们的定时对照相匹配。
