Dornbach Colten W, Broadway Paul R, Wells James E, Childress Kallie D, Thompson-Smith Aubrey C, Canterbury Landon G, Burdick Sanchez Nicole C, Mathieu Jacque, Schwarz Cory, Laverde Gomez Jenny, Tikhonova Marina, Nagaraja T G, Galyean Michael L, Hales Kristin E
Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX, United States.
USDA-ARS Livestock Issues Research Unit, Lubbock, TX, United States.
Front Microbiol. 2025 Mar 20;16:1565303. doi: 10.3389/fmicb.2025.1565303. eCollection 2025.
The objective was to longitudinally assess the prevalence of subsp. , subsp. , var, and in the nasal cavity, ruminal fluid, and feces of finishing beef steers with and without LA. Crossbred steers ( = 225; 353 ± 39.6 kg) were transported to a feedlot and fed a high-concentrate diet. Nasal, ruminal fluid, and fecal samples were collected following feedlot arrival (d 5), 1 week after adaptation to a finishing diet (d 35), and the day before harvest (study end). Livers were collected at harvest and examined for LA, and cattle were subsequently assigned into either control or liver abscess groups. Overall LA prevalence was 18.7%. The concentration and prevalence of decreased in ruminal fluid and increased in feces with days on feed ( < 0.01). Conversely, ruminal fluid prevalence of subsp. and var increased with days on feed ( < 0.01). abundance in ruminal fluid and feces was not indicative of LA development except for being more abundant in the ruminal fluid of steers with LA ( < 0.01). Abundance of subsp. was greater in abscessed liver tissue than healthy tissue ( = 0.03), although no other differences in bacterial abundance or prevalence were observed in livers. Overall, and prevalence in the nasal cavity, ruminal fluid, and feces were affected by days on feed, but their prevalence and abundance were not indicative of LA occurrence.
目的是纵向评估[具体亚种1]、[具体亚种2]、[变种]在有或无肝脓肿的育肥牛鼻腔、瘤胃液和粪便中的流行情况。杂交阉牛(n = 225;体重353 ± 39.6千克)被转运至饲养场并饲喂高浓缩日粮。在到达饲养场后第5天(d5)、适应育肥日粮1周后(d35)以及屠宰前一天(研究结束)采集鼻腔、瘤胃液和粪便样本。在屠宰时采集肝脏并检查肝脓肿情况,随后将牛分为对照组或肝脓肿组。肝脓肿总体患病率为18.7%。随着饲养天数增加,瘤胃液中[某种菌]的浓度和患病率降低,粪便中则升高(P < 0.01)。相反,瘤胃液中[具体亚种1]和[变种]的患病率随饲养天数增加而升高(P < 0.01)。瘤胃液和粪便中[某种菌]的丰度除了在有肝脓肿的阉牛瘤胃液中更丰富外(P < 0.01),并不表明肝脓肿的发生。脓肿肝脏组织中[具体亚种2]的丰度高于健康组织(P = 0.03),尽管在肝脏中未观察到细菌丰度或患病率的其他差异。总体而言,鼻腔、瘤胃液和粪便中[某种菌1]和[某种菌2]的患病率受饲养天数影响,但其患病率和丰度并不表明肝脓肿的发生。