小鼠磨牙发育过程中组蛋白乙酰化的时空分布
Temporal and spatial distribution of histone acetylation in mouse molar development.
作者信息
Du Wen, Luo Wanyi, Zheng Liwei, Zhou Xuedong, Du Wei
机构信息
State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, Department of Prosthodontics II, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
出版信息
PeerJ. 2025 Mar 31;13:e19215. doi: 10.7717/peerj.19215. eCollection 2025.
Histone acetylation is one of the most widely studied histone modification, regulating a variety of biological activities like organ development and tumorigenesis. However, the role of histone acetylation in tooth development is poorly understood. Using the mouse molar as a model, we mapped the distribution patterns of histone H3 and H4, as well as their corresponding acetylation sites during tooth formation in order to unveil the connection between histone acetylation modification and tooth development. Moreover, key histone acetyltransferases and histone deacetylases were detected in both epithelial and mesenchymal cells during tooth development by scRNA-seq and immunohistochemistry. These results suggest that histone acetylation modification functions as an important mechanism in tooth development at different stages.
组蛋白乙酰化是研究最为广泛的组蛋白修饰之一，它调控着多种生物活性，如器官发育和肿瘤发生。然而，组蛋白乙酰化在牙齿发育中的作用却鲜为人知。我们以小鼠磨牙为模型，绘制了牙齿形成过程中组蛋白H3和H4的分布模式及其相应的乙酰化位点，以揭示组蛋白乙酰化修饰与牙齿发育之间的联系。此外，通过单细胞RNA测序（scRNA-seq）和免疫组织化学方法，在牙齿发育过程中的上皮细胞和间充质细胞中检测到了关键的组蛋白乙酰转移酶和组蛋白去乙酰化酶。这些结果表明，组蛋白乙酰化修饰在牙齿发育的不同阶段发挥着重要机制的作用。
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