Anderson A W, Brydon W J, Eastwood M A, McDougall F J, Anderson D M
Food Addit Contam. 1985 Jan-Mar;2(1):33-6. doi: 10.1080/02652038509373524.
Urinary rhamnose estimations following ingestion of gum karaya were requested by the Scientific Committee for Food (EEC) in July 1983. Five male volunteers have therefore made 24-h urine collections prior to, and following, the ingestion of 10 g gum karaya for 15 days, an intake ten-fold greater than that approved in terms of the present temporary ADI (0-12 X 5 mg/kg b.w.). Paper chromatographic separations, with two solvent systems, were made on the fresh urine specimens and also after ten-fold enrichments of all urinary constituents. Standard aqueous solutions of rhamnose, and urine to which rhamnose had been added, showed the detection limit to be 0.2 microgram rhamnose. Independent examinations in two laboratories failed to detect rhamnose at this level in any of the urine specimens, Had 1% of the rhamnose present in 10 g gum karaya appeared in the 24-h urine specimens, it would have been detected. This confirms previous evidence that dietary gum karaya is neither digested nor degraded by enteric bacteria and is not absorbed to any significant extent in Man.
1983年7月，食品科学委员会（欧洲经济共同体）要求对摄入刺梧桐树胶后的尿鼠李糖进行评估。因此，五名男性志愿者在摄入10克刺梧桐树胶15天之前和之后分别进行了24小时尿液收集，该摄入量比目前临时每日允许摄入量（0 - 12×5毫克/千克体重）批准量高十倍。使用两种溶剂系统对新鲜尿液样本以及所有尿液成分进行十倍富集后进行纸色谱分离。鼠李糖标准水溶液以及添加了鼠李糖的尿液显示，检测限为0.2微克鼠李糖。两个实验室的独立检测未能在任何尿液样本中检测到该水平的鼠李糖。如果10克刺梧桐树胶中存在的1%鼠李糖出现在24小时尿液样本中，本应能检测到。这证实了先前的证据，即膳食刺梧桐树胶既不被肠道细菌消化也不被降解，并且在人体中不会被大量吸收。
