Kartha S, Felix J S, Littlefield J W
Differentiation. 1985;29(1):77-81. doi: 10.1111/j.1432-0436.1985.tb00295.x.
The components involved in cell adhesion were studied using the H6 line of embryonal carcinoma cells. H6 cells are especially suitable for studies on cell interactions, since genetic mutants can be selected, and various processes of cell adhesion can be controlled by regulating the calcium concentration in the medium. Three aggregation-defective variants of H6 were isolated, all of which showed reduced binding of the lectin, peanut agglutinin (PNA). Quantitation of PNA receptors on the cell surface by immunoprecipitation of iodinated surface proteins indicated that these receptors were reduced on the variants by one-half to one-quarter. The separation of immunoprecipitated PNA receptors on sodium-dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that one type of receptor, with an apparent molecular weight of 94 kilodaltons, was reduced. Parental and variant cells bind similar quantities of concanavalin A and soybean agglutinin, suggesting that there is no generalized effect on major glycoproteins. Thus, the defect in aggregation and the defect in the 94-kilodalton protein may be correlated, and this glycoprotein may have a role in the mediation of H6 cell-cell adhesion.
利用胚胎癌细胞H6系研究了参与细胞黏附的成分。H6细胞特别适合用于细胞相互作用的研究，因为可以选择遗传突变体，并且通过调节培养基中的钙浓度可以控制细胞黏附的各种过程。分离出了H6的三种聚集缺陷变体，所有这些变体都显示凝集素花生凝集素（PNA）的结合减少。通过对碘化表面蛋白进行免疫沉淀来定量细胞表面的PNA受体，结果表明这些受体在变体上减少了二分之一到四分之一。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上对免疫沉淀的PNA受体进行分离，结果表明一种表观分子量为94千道尔顿的受体减少了。亲本细胞和变体细胞结合的伴刀豆球蛋白A和大豆凝集素数量相似，这表明对主要糖蛋白没有普遍影响。因此，聚集缺陷和94千道尔顿蛋白的缺陷可能相关，并且这种糖蛋白可能在介导H6细胞间黏附中起作用。
