Harada H, Kochi F, Hanafusa E, Kobayashi T, Oka H, Kimura I
Gastroenterol Jpn. 1985 Feb;20(1):28-36. doi: 10.1007/BF02774671.
The effect of glucagon on human exocrine pancreatic secretion was evaluated in ten patients by analysis of pure pancreatic juice. Pancreatic juice was obtained by endoscopic cannulation of the pancreatic duct at 2-min intervals during constant intravenous infusion of secretin (1 U per kg of body weight per hr) plus caerulein (0.04 micrograms per kg of body weight per hr). Since steady secretion was established 20 minutes after the start of juice collection, a further five 2-min fractions were collected as controls, then constant intravenous infusion of glucagon (15 micrograms per kg of body weight per hr) was commenced. Pancreatic juice was collected for a further 20 minutes. The control fractions and post-glucagon fractions were compared in each patient using Student's test. Glucagon depressed secretin-caerulein-stimulated pancreatic secretions. More uniform reductions were observed in the concentration and output of protein and enzymes. Individual variations were observed in the secretory volume and bicarbonate concentration and output. Amylase and lipase were depressed in a parallel fashion in seven patients and in the remaining three, amylase was more depressed than lipase. The post-glucagon reduction in pancreatic secretion was not proportional to the rise in plasma glucagon and blood glucose.
通过对纯胰液的分析，在10名患者中评估了胰高血糖素对人胰腺外分泌的影响。在持续静脉输注促胰液素（每小时每千克体重1单位）加雨蛙肽（每小时每千克体重0.04微克）期间，每隔2分钟通过内镜插管胰管获取胰液。由于在开始收集胰液20分钟后建立了稳定分泌，因此再收集5个2分钟的样本作为对照，然后开始持续静脉输注胰高血糖素（每小时每千克体重15微克）。继续收集胰液20分钟。使用学生检验对每位患者的对照样本和胰高血糖素注射后的样本进行比较。胰高血糖素抑制了促胰液素-雨蛙肽刺激的胰腺分泌。在蛋白质和酶的浓度及分泌量方面观察到更一致的降低。在分泌量、碳酸氢盐浓度及分泌量方面观察到个体差异。在7名患者中淀粉酶和脂肪酶呈平行下降，在其余3名患者中，淀粉酶的下降比脂肪酶更明显。胰高血糖素注射后胰腺分泌的减少与血浆胰高血糖素和血糖的升高不成比例。
