Yeung W S, Chan S T
Gen Comp Endocrinol. 1985 Aug;59(2):171-83. doi: 10.1016/0016-6480(85)90367-3.
The in vitro steroidogenic capacity of the gonadal tissue in the protandrous Rhabdosargus sarba was studied. Testicular and ovarian tissues from various sexual phases were used either separately or combined. With progesterone as precursor, high yield of 5 beta-reduced metabolites, and no 11-ketotestosterone or 11 beta-hydroxytestosterone were found. The production of 5 beta-pregnan-3 alpha-ol-20-one and 5 beta-pregnane-3,20-dione was very high in incubations with testicular tissue from intersexes or males and was low in those with ovarian tissue only. The production of 17 alpha-hydroxyprogesterone was high in the female but was low in other sexual phases. With testosterone as precursor, 11-ketotestoterone and 11 beta-hydroxytestosterone were identified. The latter was the most abundant and prominent polar steroid in all incubations. 5 beta-Reductase activity was high in the male and relatively low in the female. 5 alpha-Reduced products, however, were absent. There was an increase in the production of androstenedione as the animal underwent sex reversal. The significance of this change in steroidogenesis in this protandrous fish is at present under investigation. Experimental results also indicated that in the intersexual gonad there may be interaction between the testicular component and the ovarian component in steroidgenesis.
研究了雄性先熟的条纹鲈性腺组织的体外类固醇生成能力。使用了来自不同性相的睾丸和卵巢组织，单独或组合使用。以前体酮为前体，发现5β-还原代谢产物产量高，未发现11-酮睾酮或11β-羟基睾酮。在与雌雄同体或雄性的睾丸组织孵育时，5β-孕烷-3α-醇-20-酮和5β-孕烷-3,20-二酮的产量非常高，而仅与卵巢组织孵育时产量较低。17α-羟基孕酮在雌性中的产量高，而在其他性相中的产量低。以前睾酮为前体，鉴定出了11-酮睾酮和11β-羟基睾酮。后者是所有孵育中最丰富和最突出的极性类固醇。5β-还原酶活性在雄性中高，在雌性中相对低。然而，未检测到5α-还原产物。随着动物发生性逆转，雄烯二酮的产量增加。目前正在研究这种雄性先熟鱼类类固醇生成变化的意义。实验结果还表明，在雌雄同体性腺中，睾丸成分和卵巢成分在类固醇生成中可能存在相互作用。
