Kime D E, Groves D J
Gen Comp Endocrinol. 1986 Jul;63(1):125-33. doi: 10.1016/0016-6480(86)90190-5.
Gonads of Poecilia latipinna transformed [3H]testosterone into a number of reduced and conjugated metabolites in high yield in vitro. In the male 5 beta-androstane-3 alpha, 17 beta-diol, 5 beta-androstane-3 beta, 17 beta-diol, and their "sulphates" were identified. The only 11-oxygenated androgen detected was a compound tentatively identified as 5 beta-androstane-3 beta, 11 beta, 17 beta-triol. In ovarian incubates androstenedione, 5 beta-androstane-3 alpha, 17 beta-diol and its glucuronide, testosterone glucuronide, and 5 beta-androstane-3 alpha, 17 beta-diol glucuronide were identified. Highest yields of the ovarian glucuronides coincided with the termination of vitellogenesis which may indicate a possible pheromonal role of these conjugates. In vivo plasma levels of estradiol in the female were correlated with vitellogenesis and fell markedly after castration or hypophysectomy. In males the plasma concentrations of testosterone, 11-ketotestosterone and 11 beta-hydroxytestosterone and their conjugates were variable and not apparently correlated with testicular weight, but they were reduced to undetectable levels by castration and hypophysectomy. The results suggest that 5 alpha- and 5 beta-reduced steroids may play a role in the reproductive endocrinology of P. latipinna and that measurements of only the "classical" steroid hormones in this and possibly other species may give only a partial and misleading picture of endocrine changes.
宽帆食蚊鱼的性腺在体外能将[3H]睾酮高效转化为多种还原型和结合型代谢产物。在雄性中，鉴定出了5β-雄甾烷-3α,17β-二醇、5β-雄甾烷-3β,17β-二醇及其“硫酸盐”。检测到的唯一一种11-氧化雄激素是一种暂定为5β-雄甾烷-3β,11β,17β-三醇的化合物。在卵巢孵育物中，鉴定出了雄烯二酮、5β-雄甾烷-3α,17β-二醇及其葡糖醛酸苷、睾酮葡糖醛酸苷和5β-雄甾烷-3α,17β-二醇葡糖醛酸苷。卵巢葡糖醛酸苷的最高产量与卵黄发生的终止相吻合，这可能表明这些结合物具有潜在的信息素作用。雌性体内雌二醇的血浆水平与卵黄发生相关，在去势或垂体切除后显著下降。在雄性中，睾酮、11-酮睾酮和11β-羟基睾酮及其结合物的血浆浓度各不相同，与睾丸重量没有明显相关性，但去势和垂体切除后会降至无法检测的水平。结果表明，5α-和5β-还原类固醇可能在宽帆食蚊鱼的生殖内分泌中起作用，并且仅测量该物种及可能其他物种中的“经典”类固醇激素可能只能提供内分泌变化的部分且具有误导性的情况。
