Ringenier Moniek, Cherlet Marc, Dewulf Jeroen, Devreese Mathias
Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Poult Sci. 2025 Mar 25;104(6):105098. doi: 10.1016/j.psj.2025.105098.
Fluoroquinolones (FQ) are critically important antimicrobials. When broilers are treated with FQs, residues of these compounds can accumulate in their feathers, posing potential risks if feathers are repurposed, such as for use in animal feed. The aim of this study was to determine the residue depletion profiles of enrofloxacin (ENR) and its metabolite ciprofloxacin (CIP) in broiler parent stock feathers by using a sensitive UHPLC-MS/MS method. The total antibiotic residue concentration was determined. An experimental study was conducted with a treatment group and a control group. In the treatment group, fifty broiler parent stock were administered ENR for three consecutive days at 22.5 weeks of age, with four individual feathers analysed per animal at different timepoints after administration. After 15 weeks, all animals had ENR and CIP concentrations above the limit of quantification (LOQ) of 5 ng/g feather. ENR concentrations remained relatively stable throughout the observation period. For CIP, an initial rapid decline in concentration was observed, followed by a steady-state phase. Additionally, CIP concentrations were considerably lower than those of ENR. For both ENR and CIP the within-animal variance in residue levels was higher than the variability in mean residue levels across different animals sampled at the same timepoint. In conclusion, ENR and CIP demonstrate high stability in broiler parent stock feathers and can be detected for at least 15 weeks post-treatment. These findings highlight the persisting nature of FQ residues in feathers, underscoring the need for caution when considering the reuse of feathers in agricultural or industrial applications to prevent the development of antimicrobial resistance.
氟喹诺酮类药物（FQ）是极为重要的抗菌剂。当用FQ对肉鸡进行治疗时，这些化合物的残留物会在其羽毛中蓄积，如果羽毛被重新利用，比如用于动物饲料，就会带来潜在风险。本研究的目的是通过使用灵敏的超高效液相色谱-串联质谱法（UHPLC-MS/MS）来测定恩诺沙星（ENR）及其代谢物环丙沙星（CIP）在肉种鸡羽毛中的残留消除情况。测定了总抗生素残留浓度。进行了一项实验研究，设有治疗组和对照组。在治疗组中，50只肉种鸡在22.5周龄时连续三天给予ENR，给药后在不同时间点对每只动物的四根羽毛进行分析。15周后，所有动物羽毛中的ENR和CIP浓度均高于定量限（LOQ）5 ng/g。在整个观察期内，ENR浓度保持相对稳定。对于CIP，观察到浓度最初迅速下降，随后进入稳态阶段。此外，CIP浓度明显低于ENR浓度。对于ENR和CIP，动物体内残留水平的差异均高于同一时间点不同动物平均残留水平的差异。总之，ENR和CIP在肉种鸡羽毛中表现出高稳定性，在治疗后至少15周均可检测到。这些发现突出了FQ残留物在羽毛中的持久性，强调在考虑将羽毛用于农业或工业应用时要谨慎，以防止产生抗菌药物耐药性。
